Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [3]
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Validation data
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- Product number
- PA5-55713 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- DMRT1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: LAADSASGEV GNPLGGSPVK NSLRGLPGPY VPGQTGNQWQ MKNMENRHAM SSQYRMHSYY PPPSYLGQSV PQFFTFEDAP SYPEARASVF SPPSSQDSGL VSLSSSSPIS NKSTKAVLEC EPASEPSSFT VTPVI Highest antigen sequence identity to the following orthologs: Mouse - 77%, Rat - 78%.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.2 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references 41,XX(Y) * male mice: An animal model for Klinefelter syndrome.
Wistuba J, Beumer C, Brehm R, Gromoll J
American journal of medical genetics. Part C, Seminars in medical genetics 2020 Jun;184(2):267-278
American journal of medical genetics. Part C, Seminars in medical genetics 2020 Jun;184(2):267-278
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of DMRT1 in control (vector only transfected HEK293T lysate) and DMRT1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells). Samples were probed using a DMRT1 Polyclonal Antibody (Product # PA5-55713).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of DMRT1 in human testis tissue shows strong nuclear positivity in fraction of cells in seminiferus ducts. Samples were probed using a DMRT1 Polyclonal Antibody (Product # PA5-55713).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of DMRT1 in human testis and fallopian tube tissues using DMRT1 Polyclonal Antibody (Product # PA5-55713). Corresponding DMRT1 RNA-seq data are presented for the same tissues.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of DMRT1 in human fallopian tube using DMRT1 Polyclonal Antibody (Product # PA5-55713) shows low expression as expected.