Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- AF4077 - Provider product page
- Provider
- R&D Systems
- Product name
- Human TFF2 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human TFF2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant human TFF3 is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
Q03403
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Detection of Human TFF2 by Western Blot. Western blot shows lysates of human stomach tissue, human stomach cancer tissue, human breast tissue, and human kidney tissue. PVDF membrane was probed with 2 µg/mL of Goat Anti-Human TFF2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4077) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for TFF2 at approximately 19-21 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.