Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Flow cytometry [1]
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Validation data
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- Product number
- GTX79231 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX79231, RRID:AB_11178090
- Product name
- CNG Channel antibody [CNC 9C1]
- Antibody type
- Monoclonal
- Host
- Mouse
- Vial size
- 100µl
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Western blot analysis of TCR V delta 2 in 25 μg of Jurkat and CEM cell lysates. Proteins were transferred to a PVDF membrane and blocked at 4°C overnight. The membrane was probed with TCR V delta 2 antibody [15D] at a dilution of 1:10 overnight at 4°C, washed in TBST, and probed with an HRP-conjμgated secondary antibody. Chemiluminescent detection was performed.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Flow cytometry analysis of TCR V delta 2 in Jurkat cells (green) compared to an isotype control (blue). Cells were harvested, adjusted to a concentration of 1-5x10^6 cells/ml, fixed with 2% paraformaldehyde and washed with PBS. Cells were blocked with a 2% solution of BSA-PBS for 30 min at room temperature and incubated with TCR V delta 2 antibody [15D] at a dilution of 2 μg/test for 60 min at room temperature. Cells were then probe with a proper secondary antibody and re-suspended in PBS for FACS analysis.