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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
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Validation data
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- Product number
- PA5-21903 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PITPNM3 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Recommended positive controls: MCF-7, MDA-MB-231. Predicted reactivity: Mouse (100%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of NIR1 using 30 µg of A) 293T and B) Raji lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a NIR1 polyclonal antibody (Product # PA5-21903) at a dilution of 1:1500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of PITPNM3 was performed by separating 30 µg of various whole cell extracts by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a PITPNM3 Polyclonal Antibody (Product # PA5-21903) at a dilution of 1:500 and a HRP-conjugated anti-rabbit IgG secondary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- PITPNM3 Polyclonal Antibody detects PITPNM3 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in -20ºC 100% MeOH for 5 min. Green: PITPNM3 protein stained by PITPNM3 Polyclonal Antibody (Product # PA5-21903) diluted at 1:500. Blue: Hoechst 33343 staining.
