PA5-47487

antibody from Invitrogen Antibodies

Targeting: PTPRS

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-47487

Provider

Invitrogen Antibodies

Product name

PTPRS Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

This antibody detects the endogenous cleaved extracellular domain of human PTP sigma /PTPRS in Western blots. In Western blots, less than 1% cross-reactivity with recombinant human LAR, PTPR beta, PTPR gamma, PTPR mu, or PTPR kappa is observed. Reconstitute at 0.2 mg/mL in sterile PBS.

Reactivity

Human

Host

Goat

Isotype

IgG

Vial size

100 µg

Concentration

0.2 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of PTPRS in SH-SY5Y human neuroblastoma cell line, CHP-100 human neuroblastoma cell line, and A431 human epithelial carcinoma cell line. Samples were incubated in PTPRS polyclonal antibody (Product # PA5-47487) using a dilution of 1 µg/mL followed by a HRP-conjugated Anti-Goat IgG secondary antibody. For additional reference, recombinant human PTPRµ, PTPRκ, LAR, PTPRσ, PTPRδ, and PTPRβ (40 ng/lane) were included. A specific band for PTPσ/PTPRS was detected at approximately 140 kDa (as indicated). This experiment was conducted under reducing conditions.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry analysis of PTPRS in immersion fixed A431 human epithelial carcinoma cell line. Samples were incubated in PTPRS polyclonal antibody (Product # PA5-47487) using a dilution of 10 µg/mL for 3 hours at room temperature followed by NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red) and counterstained with DAPI(blue). Specific staining was localized to cytoplasm.