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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- A301-273A - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- RCOR3 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.20 mg/mL
- Storage
- 4° C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human and mouse RCOR3 by western blot (h&m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 µg) and mouse NIH3T3 (M; 50 µg) cells. Antibodies: Affinity purified rabbit anti-RCOR3 antibody A301-273A used for WB at 0.04 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate. RCOR3 was also immunoprecipitated by rabbit anti-RCOR3 antibody A301-272A, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 1 second (A) and 10 seconds (B).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human RCOR3 by immunocytochemistry. Sample: FFPE section of NBF-fixed HeLa Cells Antibody: Affinity purified rabbit anti- RCOR3 (Cat. No. A300-273A Lot1) used at a dilution of 1:80 (2.5 µg/mL). Detection: Green-fluorescent goat anti-rabbit IgG-heavy and light chain cross-adsorbed Antibody DyLight® 488 Conjugated used at a dilution of 1:100.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human RCOR3 by immunohistochemistry. Sample: FFPE sections of human testicular seminoma (left) and breast carcinoma (right). Antibody: Affinity purified rabbit anti- RCOR3 (Cat. No. A301-273A Lot1) used at a dilution of 1:200 (1µg/ml) and 1:80 (2.5 µg/ml). Detection: DAB and Red-fluorescent goat anti-rabbit IgG-heavy and light chain, cross-adsorbed Antibody DyLight® 594 Conjugated used at a dilution of 1:100.
