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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- NB100-60637 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-60637, RRID:AB_2177999
- Product name
- Rabbit Polyclonal CoREST3/RCOR3 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 0.2 mg/ml
- Storage
- Store at 4C. Do not freeze.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: CoREST3/RCOR3 Antibody [NB100-60637] - Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug) and mouse NIH3T3 (M; 50 ug) cells. NB100-60637 used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. RCOR3 was also immunoprecipitated by rabbit anti-RCOR3 antibody NB100-60636, which recognizes an upstream epitope.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: CoREST3/RCOR3 Antibody [NB100-60637] - Sample: FFPE sections of human testicular seminoma (left) and breast carcinoma (right). Antibody: Affinity purified rabbit anti- RCOR3 used at a dilution of 1:200 (1ug/ml) and 1:80 (2.5 ug/ml). Detection: DAB and Red-fluorescent Goat anti-Rabbit IgG-heavy
