PA5-98222

antibody from Invitrogen Antibodies

Targeting: TXK BTKL, PSCTK5, PTK4, RLK, TKL

Western blot (Data presented on provider website)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-98222

Provider

Invitrogen Antibodies

Product name

TXK Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Reactivity

Human, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µg

Concentration

3 mg/mL

Storage

-20°C or -80°C if preferred

References

Submitted references

Effects of Txk‑mediated activation of NF‑κB signaling pathway on neurological deficit and oxidative stress after ischemia‑reperfusion in rats.

Xu QL, Wu J
Molecular medicine reports 2021 Jul;24(1)

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of TXK in HepG2 cells using a TXK polyclonal antibody (Product # PA5-98222) at a dilution of 1:100. Alexa Fluor 488-congugated Goat Anti-Rabbit IgG(H+L) secondary antibody was used.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of TXK in paraffin embedded human breast cancer using a TXK polyclonal antibody (Product # PA5-98222) at a dilution of 1:100.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of TXK in paraffin embedded human lymph node tissue using a TXK polyclonal antibody (Product # PA5-98222) at a dilution of 1:100.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 1. Western blotting and immunofluorescence results. (A) Western blot analysis of Txk expression in sham and MCAO 6-, 12-, 24- and 48-h groups. (B) Quantification of Txk in different groups. (C) Western blot analysis of Txk expression in control and OGD/R 1-, 2-, 6- and 12-h neurons. (D) Quantification of Txk in different neurons. (E) Immunofluorescence assay of Txk in sham and MCAO rats. Magnification, x400) (F) Immunofluorescence assay of Txk in control and OGD/R neurons. Magnification, x1000 (G) Txk-positive cells in sham and MCAO rats and (H) fluorescence intensity in control and OGD/R neurons. Protein level was normalized to beta-actin. *P