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- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Immunocytochemistry [2]
- Immunohistochemistry [4]
- Other assay [1]
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- Product number
- PA5-56644 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- FRYL Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Immunogen sequence: AYCKLINQVN TIKNEAEVIN MSEELAQLES ILKEAESASE NEEIDISKAA QTTIETAIHS LIETLKNKEF ISAVAQVKA Highest antigen sequence identity to the following orthologs: Mouse - 96%, Rat - 92%.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.2 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references MicroRNA-1205 Regulation of FRYL in Prostate Cancer.
Naidoo M, Levine F, Gillot T, Orunmuyi AT, Olapade-Olaopa EO, Ali T, Krampis K, Pan C, Dorsaint P, Sboner A, Ogunwobi OO
Frontiers in cell and developmental biology 2021;9:647485
Frontiers in cell and developmental biology 2021;9:647485
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of FRYL in human cell line U-251 MG shows positivity in cytoplasm & microtubules. Samples were probed using a FRYL Polyclonal Antibody (Product # PA5-56644).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorecent analysis of FRYL in human cell line U-251 MG using FRYL Polyclonal Antibody (Product # PA5-56644). Staining shows localization to cytosol and microtubules.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of FRYL in human cerebral cortex using FRYL Polyclonal Antibody (Product # PA5-56644) shows moderate cytoplasmic positivity in neuropil.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of FRYL in human duodenum using FRYL Polyclonal Antibody (Product # PA5-56644) shows strong positivity in apical membrane in glandular cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of FRYL in human fallopian tube using FRYL Polyclonal Antibody (Product # PA5-56644) shows moderate positivity in cilia in glandular cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of FRYL in human liver using FRYL Polyclonal Antibody (Product # PA5-56644) shows no positivity in hepatocytes as expected.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- FIGURE 4 FRYL is overexpressed in PCa and is regulated by miR-1205. (A) FRYL was significantly overexpressed in PCa tissues when compared to normal tissues {ANOVA [ F (1, 22) = 4.3, p = 0.009]. (B) Using thesame cohort of tissues in Figure 1A , FRYL overexpression was observed in PCa tissues when compared to normal prostatic tissues. (C) Western blot analysis of FRYL in CRPC C4-2B, small cell prostate carcinoma PC-3 cells and androgen-sensitive LNCaP cells. (D) FRYL protein levels in PC-3 cells after inhibition of miR-1205 with NB1205. Data is presented as +- SD.
