Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [6]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA5-53277 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- EQTN Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: SCESQYSVNP ELATMSYFHP SEGVSDTSFS KSAESSTFLG TTSSDMRRSG TRTSESKIMT DIISIGSDNE MHENDESVTR Highest antigen sequence identity to the following orthologs: Mouse - 61%, Rat - 33%.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of EQTN in control (vector only transfected HEK293T lysate) and EQTN over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells). Samples were probed using a EQTN Polyclonal Antibody (Product # PA5-53277).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of EQTN in human testis and prostate tissues using EQTN Polyclonal Antibody (Product # PA5-53277). Corresponding EQTN RNA-seq data are presented for the same tissues.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of EQTN in human colon, liver, prostate and testis using EQTN Polyclonal Antibody (Product # PA5-53277) (A) shows similar protein distribution across tissues to an independent EQTN Polyclonal Antibody (B).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of EQTN in human prostate using EQTN Polyclonal Antibody (Product # PA5-53277) shows no positivity in glandular cells as expected.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of EQTN in human testis using EQTN Polyclonal Antibody (Product # PA5-53277) shows strong positivity in cells in seminiferous ducts.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of EQTN in human colon using EQTN Polyclonal Antibody (Product # PA5-53277) shows no positivity in glandular cells as expected.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of EQTN in human liver using EQTN Polyclonal Antibody (Product # PA5-53277) shows no positivity in hepatocytes as expected.
