PA5-53277

antibody from Invitrogen Antibodies

Targeting: EQTN AFAF, C9orf11, equatorin, SPACA8

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-53277

Provider

Invitrogen Antibodies

Product name

EQTN Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: SCESQYSVNP ELATMSYFHP SEGVSDTSFS KSAESSTFLG TTSSDMRRSG TRTSESKIMT DIISIGSDNE MHENDESVTR

Concentration

0.1 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of EQTN in control (vector only transfected HEK293T lysate) and EQTN over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells). Samples were probed using a EQTN Polyclonal Antibody (Product # PA5-53277).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of EQTN in human testis and prostate tissues using EQTN Polyclonal Antibody (Product # PA5-53277). Corresponding EQTN RNA-seq data are presented for the same tissues.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of EQTN in human colon, liver, prostate and testis using EQTN Polyclonal Antibody (Product # PA5-53277) (A) shows similar protein distribution across tissues to an independent EQTN Polyclonal Antibody (B).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of EQTN in human prostate using EQTN Polyclonal Antibody (Product # PA5-53277) shows no positivity in glandular cells as expected.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of EQTN in human testis using EQTN Polyclonal Antibody (Product # PA5-53277) shows strong positivity in cells in seminiferous ducts.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of EQTN in human colon using EQTN Polyclonal Antibody (Product # PA5-53277) shows no positivity in glandular cells as expected.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of EQTN in human liver using EQTN Polyclonal Antibody (Product # PA5-53277) shows no positivity in hepatocytes as expected.