Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [6]
Submit
Validation data
Reference
Comment
Report error
- Product number
- NBP1-90869 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-90869, RRID:AB_11003258
- Product name
- Rabbit Polyclonal EQTN Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specificity of human C9orf11 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Contribution of antibody-based protein profiling to the human Chromosome-centric Proteome Project (C-HPP).
Fagerberg L, Oksvold P, Skogs M, Algenäs C, Lundberg E, Pontén F, Sivertsson A, Odeberg J, Klevebring D, Kampf C, Asplund A, Sjöstedt E, Al-Khalili Szigyarto C, Edqvist PH, Olsson I, Rydberg U, Hudson P, Ottosson Takanen J, Berling H, Björling L, Tegel H, Rockberg J, Nilsson P, Navani S, Jirström K, Mulder J, Schwenk JM, Zwahlen M, Hober S, Forsberg M, von Feilitzen K, Uhlén M
Journal of proteome research 2013 Jun 7;12(6):2439-48
Journal of proteome research 2013 Jun 7;12(6):2439-48
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: C9orf11 Antibody [NBP1-90869] - Analysis in control (vector only transfected HEK293T lysate) and EQTN over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: C9orf11 Antibody [NBP1-90869] - Staining of human liver shows no positivity in hepatocytes as expected.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: C9orf11 Antibody [NBP1-90869] - Staining of human colon shows no positivity in glandular cells as expected.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: C9orf11 Antibody [NBP1-90869] - Staining of human colon, liver, prostate and testis using Anti-EQTN antibody NBP1-90869 (A) shows similar protein distribution across tissues to independent antibody NBP1-90868 (B).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: C9orf11 Antibody [NBP1-90869] - Analysis in human testis and prostate tissues. Corresponding EQTN RNA-seq data are presented for the same tissues.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: C9orf11 Antibody [NBP1-90869] - Staining of human testis shows strong positivity in cells in seminiferous ducts.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: C9orf11 Antibody [NBP1-90869] - Staining of human prostate shows no positivity in glandular cells as expected.