Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PAB12307 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB12307, RRID:AB_10677443
- Product name
- XAF1 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of XAF1.
- Isotype
- IgG
- Storage
- Store at -20°C on dry atmosphere.After reconstitution with 200 uL of deionized water and concentration will be 500 ug/mL, store at -20°C or lower.Aliquot to avoid repeated freezing and thawing.
Submitted references Identification of XAF1 as an antagonist of XIAP anti-Caspase activity.
Expression and genetic analysis of XIAP-associated factor 1 (XAF1) in cancer cell lines.
Liston P, Fong WG, Kelly NL, Toji S, Miyazaki T, Conte D, Tamai K, Craig CG, McBurney MW, Korneluk RG
Nature cell biology 2001 Feb;3(2):128-33
Nature cell biology 2001 Feb;3(2):128-33
Expression and genetic analysis of XIAP-associated factor 1 (XAF1) in cancer cell lines.
Fong WG, Liston P, Rajcan-Separovic E, St Jean M, Craig C, Korneluk RG
Genomics 2000 Nov 15;70(1):113-22
Genomics 2000 Nov 15;70(1):113-22
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western Blot analysis of XAF1 expression from cell and tissue extracts with XAF1 polyclonal antibody (Cat # PAB12307).Lane 1 : rat liver tissue lysate.Lane 2 : rat kidney tissue lysate.Lane 3 : rat spleen tissue lysate.Lane 4 : human placenta tissue lysate.Lane 5 : Marker.Lane 6 : MCF-7 whole cell lysate.Lane 7 : HeLa whole cell lysate.Lane 8 : smmc cell lysate.Lane 9 : HT1080 whole cell lysate.Lane 10 : Colo 320 whole cell lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunohistochemical staining of XAF1 on formalin fixed, paraffin embedded human epidermic carcinoma with XAF1 polyclonal antibody (Cat # PAB12307).
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)