Explore
Validate
Learn
Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- MA5-35585 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- DNAJC15 Recombinant Rabbit Monoclonal Antibody (7D7X7)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Description
- Immunogen sequence: ETAKKISTPS FSSYYKGGFE QKMSRREAGL ILGVSPSAGK AKIRTAHRRV MILNHPDKGG SPYVAAKINE AKDLLETTTK H
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- 7D7X7
- Vial size
- 100 µL
- Concentration
- 0.59 mg/mL
- Storage
- -20°C, Avoid Freeze/Thaw Cycles
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of DNAJC15 in extracts of various cell lines. Samples were incubated with DNAJC15 Monoclonal antibody (Product # MA5-35585) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of DNAJC15 in NIH-3T3 cells. Samples were incubated with DNAJC15 Monoclonal antibody (Product # MA5-35585) using a dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
