F54425

antibody from NSJ Bioreagents

Targeting: HPX

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

F54425

Provider

NSJ Bioreagents

Product name

HPX Antibody / Hemopexin

Antibody type

Polyclonal

Description

This highly specific HPX antibody is suitable for use in Immunohistochemistry/Western blot/Flow cytometry applications with human, mouse and rat samples.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Vial size

0.08 ml, 0.4 ml

Concentration

In 1X PBS, pH 7.4, with 0.09% sodium azide

Storage

Aliquot the HPX antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of 1) human HepG2, 2) human plasma, 3) mouse plasma and 4) rat plasma lysate with HPX antibody. Predicted molecular weight: 52-75 kDa depending on glycosylation level.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of 1) human liver and 2) rat liver lysate with HPX antibody. Predicted molecular weight: 52-75 kDa depending on glycosylation level.

Immunohistochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC testing of FFPE human liver tissue with HPX antibody. HIER: steam section in pH9 EDTA for 20 min and allow to cool prior to staining.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC testing of FFPE human liver tissue with HPX antibody. HIER: steam section in pH9 EDTA for 20 min and allow to cool prior to staining.

Flow cytometry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Flow cytometry testing of human CCRF-CEM cells with HPX antibody; Blue=isotype control, Green= HPX antibody.