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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Immunocytochemistry [2]
- Other assay [1]
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- Product number
- PA5-20834 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- OCIAD1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- A suggested positive control is 293 cell lysate.
- Concentration
- 1 mg/mL
Submitted references Genome-wide CRISPRi screening identifies OCIAD1 as a prohibitin client and regulatory determinant of mitochondrial Complex III assembly in human cells.
Le Vasseur M, Friedman J, Jost M, Xu J, Yamada J, Kampmann M, Horlbeck MA, Salemi MR, Phinney BS, Weissman JS, Nunnari J
eLife 2021 May 26;10
eLife 2021 May 26;10
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of OCIAD1 in 293 cell lysate with OCIAD1 Polyclonal Antibody (Product # PA5-20834) at (A) 1 and (B) 2 µg/mL.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence of OCIAD1 in 293 cells with OCIAD1 Polyclonal Antibody (Product # PA5-20834) at 20 µg/mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence of OCIAD1 in 293 cells with OCIAD1 Polyclonal Antibody (Product # PA5-20834) at 20 µg/mL.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 3. Ovarian carcinoma immunoreactive antigen domain-containing protein 1 (OCIAD1) is an inner mitochondrial membrane protein. ( A ) Schematic illustration of OCIAD1 domain organization. ( B ) Representative images of fixed U2OS cells stained with Mitotracker (magenta) and immunolabeled using anti-OCIAD1 antibodies (green). Lower panel is a magnification of the inset shown in the upper panel. ( C ) OCIAD1 is an integral membrane protein. Sodium carbonate extraction fractions (pH 10.5-11.5) immunoblotted with anti-OCIAD1, anti-TIM50, anti-ATP5A1, and anti-SDHA antibodies. P and S indicate pellet and soluble fractions, respectively. ( D ) OCIAD1 localizes to the inner membrane. Protease protection assay fractions immunoblotted with anti-OCIAD1, anti-prohibitin 2 (PHB2), anti-TIM50, anti-ATP5A1, and anti-SDHA antibodies. (OMM: outer mitochondrial membrane, IMM: inner mitochondrial membrane, IMS: intermembrane space). ( E ) Schematic illustration of OCIAD1 topology within the inner membrane. Figure 3--figure supplement 1. Ovarian carcinoma immunoreactive antigen domain-containing protein 1 (OCIAD1) termini are localized in the mitochondrial intermembrane space. ( A ) Schematic illustrating the OCIAD1-GFP deletion constructs used for mapping the epitope of the anti-OCIAD1 polyclonal antibody. ( B ) Cell lysates from U2OS cells expressing either full-length or truncated OCIAD1-GFP were analyzed by Western blotting and immunoprobed using anti-OCIAD1 (Invitrogen, cat# PA5-20834)
