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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [6]
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- Product number
- PA5-59375 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- OCIAD2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: GVCQSKFHFF EDQLRGAGFG PQHNRHCLLT CEECKIKHGL SEKGDSQPSA S Highest antigen sequence identity to the following orthologs: Mouse - 82%, Rat - 43%.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Genome-wide CRISPRi screening identifies OCIAD1 as a prohibitin client and regulatory determinant of mitochondrial Complex III assembly in human cells.
Le Vasseur M, Friedman J, Jost M, Xu J, Yamada J, Kampmann M, Horlbeck MA, Salemi MR, Phinney BS, Weissman JS, Nunnari J
eLife 2021 May 26;10
eLife 2021 May 26;10
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of OCIAD2 using OCIAD2 Polyclonal Antibody (Product # PA5-59375) (A) shows similar pattern to an independent OCIAD2 Polyclonal Antibody (B).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of OCIAD2 in human cell lines U-251MG and Caco-2 using a OCIAD2 Polyclonal Antibody (Product # PA5-59375). Corresponding OCIAD2 RNA-seq data are presented for the same cell lines. Loading control: Anti-GAPDH.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of OCIAD2 in human cell line U-251 MG shows positivity in mitochondria. Samples were probed using an OCIAD2 Polyclonal Antibody (Product # PA5-59375).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of OCIAD2 in human stomach using OCIAD2 Polyclonal Antibody (Product # PA5-59375).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of OCIAD2 in human liver using OCIAD2 Polyclonal Antibody (Product # PA5-59375).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of OCIAD2 in human kidney tissue shows strong cytoplasmic positivity in tubular cells. Samples were probed using an OCIAD2 Polyclonal Antibody (Product # PA5-59375).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of OCIAD2 in human kidney and skeletal muscle tissues using OCIAD2 Polyclonal Antibody (Product # PA5-59375). Corresponding OCIAD2 RNA-seq data are presented for the same tissues.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of OCIAD2 in human kidney, liver, skeletal muscle and stomach using OCIAD2 Polyclonal Antibody (Product # PA5-59375) (A) shows similar protein distribution across tissues to an independent OCIAD2 Polyclonal Antibody (B).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of OCIAD2 in human skeletal muscle using OCIAD2 Polyclonal Antibody (Product # PA5-59375) shows low expression as expected.
