PA5-59375

antibody from Invitrogen Antibodies

Targeting: OCIAD2 MGC45416

Western blot (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-59375

Provider

Invitrogen Antibodies

Product name

OCIAD2 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Immunogen sequence: GVCQSKFHFF EDQLRGAGFG PQHNRHCLLT CEECKIKHGL SEKGDSQPSA S Highest antigen sequence identity to the following orthologs: Mouse - 82%, Rat - 43%.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Genome-wide CRISPRi screening identifies OCIAD1 as a prohibitin client and regulatory determinant of mitochondrial Complex III assembly in human cells.

Le Vasseur M, Friedman J, Jost M, Xu J, Yamada J, Kampmann M, Horlbeck MA, Salemi MR, Phinney BS, Weissman JS, Nunnari J
eLife 2021 May 26;10

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of OCIAD2 in human cell line U-251 MG shows positivity in mitochondria. Samples were probed using an OCIAD2 Polyclonal Antibody (Product # PA5-59375).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorecent analysis of OCIAD2 in human cell line U-251 MG using OCIAD2 Polyclonal Antibody (Product # PA5-59375). Staining shows localization to mitochondria.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of OCIAD2 in human kidney using OCIAD2 Polyclonal Antibody (Product # PA5-59375) shows strong granular cytoplasmic positivity in cells in tubules.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of OCIAD2 in human kidney and skeletal muscle tissues using OCIAD2 Polyclonal Antibody (Product # PA5-59375). Corresponding OCIAD2 RNA-seq data are presented for the same tissues.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of OCIAD2 in human kidney, liver, skeletal muscle and stomach using OCIAD2 Polyclonal Antibody (Product # PA5-59375) (A) shows similar protein distribution across tissues to an independent OCIAD2 Polyclonal Antibody (B).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of OCIAD2 in human skeletal muscle using OCIAD2 Polyclonal Antibody (Product # PA5-59375) shows low expression as expected.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of OCIAD2 in human liver using OCIAD2 Polyclonal Antibody (Product # PA5-59375).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of OCIAD2 in human stomach using OCIAD2 Polyclonal Antibody (Product # PA5-59375).