14651-1-AP

antibody from Invitrogen Antibodies

Targeting: TMPO LAP2, LEMD4, TP

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

14651-1-AP

Provider

Invitrogen Antibodies

Product name

LAP2 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: MPEFLEDPS VLTKDKLKSE LVANNVTLPA GEQRKDVYVQ LYLQHLTARN RPPLPAGTNS KGPPDFSSDE EREPTPVLGS GAAAAGRSRA AVGRKATKKT DKPRQEDKDD LDVTELTNED LLDQLVKYGV NPGPIVGTTR KLYEKKLLKL REQGTESRSS TPLPTISSSA ENTRQNGSND SDRYSDNEED SKIELKLEKR EPLKGRAKTP VTLKQRRVEH NQSYSQAGIT ETEWTSGSSK GGPLQALTRE STRGSRRTPR KRVETSEHFR IDGPVISEST PIAETIMASS NESLVVNRVT GNFKHASPIL PITEFSDIPR RAPKKPLTRA EVGEKTEERR VERDILKEMF PYEASTPTGI SAS (1-362 aa encoded by BC053675)

Reactivity

Human, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.13 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

K-562 cells were subjected to SDS PAGE followed by western blot with 14651-1-AP (LAP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HeLa cells were subjected to SDS PAGE followed by western blot with 14651-1-AP (LAP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IF result of anti-TMPO (14651-1-AP) in Hela cell by Dr. Ralph Kehlenbach.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TMPO/LAP2 antibody (Product # 14651-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human skin cancer using 14651-1-AP (LAP2 antibody) at dilution of 1:100 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human skin cancer using 14651-1-AP (LAP2 antibody) at dilution of 1:100 (under 40x lens).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-LAP2 (IP:14651-1-AP, 4ug; Detection:14651-1-AP 1:500) with HeLa cells lysate 1600ug.