NBP1-87822

antibody from Novus Biologicals

Targeting: TMPO LAP2, LEMD4, TP

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

NBP1-87822

Provider

Novus Biologicals

Proper citation

Novus Cat#NBP1-87822, RRID:AB_11021159

Product name

Rabbit Polyclonal Thymopoietin/LAP2 Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. Specificity of human Thymopoietin/LAP2 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

0.1 ml

Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

References

Submitted references

Long noncoding RNA TMPO-AS1 promotes progression of non-small cell lung cancer through regulating its natural antisense transcript TMPO.

Qin Z, Zheng X, Fang Y
Biochemical and biophysical research communications 2019 Aug 20;516(2):486-493

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Systematic validation of antibody binding and protein subcellular localization using siRNA and confocal microscopy.

Stadler C, Hjelmare M, Neumann B, Jonasson K, Pepperkok R, Uhlén M, Lundberg E
Journal of proteomics 2012 Apr 3;75(7):2236-51

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: Thymopoietin/LAP2 Antibody [NBP1-87822] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: Thymopoietin/LAP2 Antibody [NBP1-87822] - Analysis in U2OS cells transfected with control siRNA, target specific siRNA probe #1 and #2, using anti-TMPO antibody. Remaining relative intensity is presented.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: Thymopoietin/LAP2 Antibody [NBP1-87822] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Thymopoietin/LAP2 Antibody [NBP1-87822] - Staining of human lymph node shows high expression.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Thymopoietin/LAP2 Antibody [NBP1-87822] - Staining of human pancreas shows low expression as expected.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Thymopoietin/LAP2 Antibody [NBP1-87822] - Staining of human lymph node shows strong positivity in nuclear membrane in germinal center cells.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Thymopoietin/LAP2 Antibody [NBP1-87822] - Staining of human prostate shows strong positivity in nuclear membrane in glandular cells.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Thymopoietin/LAP2 Antibody [NBP1-87822] - Staining of human skeletal muscle shows moderate positivity in nuclear membrane in a small subset of myocytes.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Thymopoietin/LAP2 Antibody [NBP1-87822] - Staining of human small intestine shows strong positivity in nuclear membrane in glandular cells.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Thymopoietin/LAP2 Antibody [NBP1-87822] - Staining in human lymph node and skeletal muscle tissues using NBP1-87822 antibody. Corresponding TMPO RNA-seq data are presented for the same tissues.