Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [2]
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Validation data
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- Product number
- GTX115949 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX115949, RRID:AB_10621536
- Product name
- GCP4 antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse
- Host
- Rabbit
Submitted references MZT1 regulates microtubule nucleation by linking γTuRC assembly to adapter-mediated targeting and activation.
The dynamics of microtubule minus ends in the human mitotic spindle.
Cota RR, Teixidó-Travesa N, Ezquerra A, Eibes S, Lacasa C, Roig J, Lüders J
Journal of cell science 2017 Jan 15;130(2):406-419
Journal of cell science 2017 Jan 15;130(2):406-419
The dynamics of microtubule minus ends in the human mitotic spindle.
Lecland N, Lüders J
Nature cell biology 2014 Aug;16(8):770-8
Nature cell biology 2014 Aug;16(8):770-8
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- GCP4 antibody detects TUBGCP4 protein by Western blot analysis.A. 30 ?g NIH-3T3 whole cell lysate/extract 10 % SDS-PAGEGCP4 antibody (GTX115949) dilution: 1:1000
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed 293T, using GCP4(GTX115949) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:500.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- GCP4 antibody detects GCP4 protein at cytoplasm and centrosome by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: GCP4 protein stained by GCP4 antibody (GTX115949) diluted at 1:1000.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.Blue: Hoechst 33342 staining.