A301-379A

antibody from Invitrogen Antibodies

Targeting: AKAP1 AKAP121, AKAP149, AKAP84, D-AKAP1, PPP1R43, PRKA1, S-AKAP84, SAKAP84, TDRD17

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

A301-379A

Provider

Invitrogen Antibodies

Product name

AKAP1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.20 mg/mL

Storage

4° C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human AKAP1 by western blot and immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-AKAP1 antibody A301-379A used for WB at 0.04 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate. AKAP1 was also immunoprecipitated by rabbit anti-AKAP1 antibodies A301-380A and BL5622, which recognize downstream epitopes. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 3 seconds (B).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human AKAP1 by immunohistochemistry. Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-AKAP1 (Cat. No. A301-379A) used at a dilution of 1:100 ( 2µg/ml). Detection: Red-fluorescent goat anti-rabbit IgG highly cross-adsorbed Antibody.