PAB22193

antibody from Abnova Corporation

Targeting: GEMIN8 FAM51A1, FLJ20514

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB22193

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB22193, RRID:AB_10963718

Product name

GEMIN8 polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against recombinant GEMIN8.

Antigen sequence

ASTSKATRPWYSHPVYARYWQHYHQAMAWMQSHHN
AYRKAVESCFNLPWYLPSALLPQSSYDNEAAYPQS
FYDHHVAWQDYPCSSSHFRRSGQHPRYSSRIQAST
KEDQALSKEEEMETESDAEVECDLSNMEITEELRQ
YFAETER


Isotype

IgG

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Lane 1: RT-4, Lane 2: U-251 MG, Lane 3: Human Plasma, Lane 4: Liver, Lane 5: Tonsil with GEMIN8 polyclonal antibody (Cat # PAB22193) at 1:250-1:500 dilution.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line U-2 OS with GEMIN8 polyclonal antibody (Cat # PAB22193) at 1-4 ug/mL dilution shows positivity in nucleus but not nucleoli and cytoplasm.

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of human colon with GEMIN8 polyclonal antibody (Cat # PAB22193) shows moderate cytoplasmic positivity in glandular cells at 1:20-1:50 dilution.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)