PA5-89189

antibody from Invitrogen Antibodies

Targeting: ELOB SIII, TCEB2

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-89189

Provider

Invitrogen Antibodies

Product name

TCEB2 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: MDVFLMIRRH KTTIFTDAKE SSTVFELKRI VEGILKRPPD EQRLYKDDQL LDDGKTLGEC GFTSQTARPQ APATVGLAFR ADDTFEALCI EPFSSPPELP DVMKPQDSGS SANEQAVQ; Positive Samples: 22Rv1, NCI-H460, MCF7; Cellular Location: Nucleus

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1.07 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of extracts of various cell lines, using TCEB2 Polyclonal antibody (Product # PA5-89189) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Exposure time: 90s.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of TCEB2 in extracts of various cell lines using TCEB2 Polyclonal Antibody (Product # PA5-89189) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of TCEB2 in paraffin-embedded human colon using TCEB2 Polyclonal Antibody (Product # PA5-89189) at a dilution of 1:200.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of TCEB2 in paraffin-embedded human esophagus using TCEB2 Polyclonal Antibody (Product # PA5-89189) at a dilution of 1:100.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of TCEB2 in paraffin-embedded human stomach using TCEB2 Polyclonal Antibody (Product # PA5-89189) at a dilution of 1:100.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoprecipitation analysis of TCEB2 was performed in 150 µg extracts of MCF7 cells using TCEB2 Polyclonal Antibody (Product # PA5-89189). Western blot was performed from the immunoprecipitate using TCEB2 Polyclonal Antibody.