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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- 629304 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#629304, RRID:AB_2303139
- Product name
- Biotin anti-Elongin B
- Antibody type
- Monoclonal
- Antigen
- Full length recombinant protein
- Description
- Elongin B (also known as RNA polymerase II transcription factor SIII p18 subunit and transcription elongation factor B polypeptide 2) is an 18 kD member of the UbH family containing a ubiquitin-like domain. Elongin functions as a subunit of a general transcription elongation factor that increases RNA polymerase II transcription elongation past template-encoded arresting sites in the nucleus. The Elongin BC complex acts as adaptor to link Elongin A, VHL, WSB1 or SOCS1 with a module of CUL2 or CUL5 and RBX1 to form E3 ubiquitin ligases. The 10C4 monoclonal antibody recognizes the human elongin B protein and has been shown to be useful for Western blotting.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Biotin
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 0.5 mg/ml
- Storage
- The antibody solution should be stored undiluted at 4°C. Do not freeze.
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Supportive validation
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- BioLegend (provider)
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- Experimental details
- Hela cell nuclear extracts were resolved by electrophoresis, transferred to nitrocellulose and probed with monoclonal anti-elongin B (clone 10C4). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence system.
- Conjugate
- Biotin
