11453-1-AP

antibody from Proteintech Group

Targeting: FERMT2 KIND2, mig-2, PLEKHC1, UNC112B

Provider product page for 11453-1-AP

Western blot

ELISA

Immunocytochemistry

Immunoprecipitation

Immunohistochemistry

Antibody data

Antibody Data
Antigen structure
References [44]
Comments [0]
Validations
Western blot [1]
Immunocytochemistry [1]
Immunoprecipitation [1]
Immunohistochemistry [1]

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Product number: 11453-1-AP - Provider product page
Provider: Proteintech Group
Proper citation: Proteintech Cat#11453-1-AP, RRID:AB_2262660
Product name: Kindlin 2 antibody
Antibody type: Polyclonal
Description: KD/KO validated Kindlin 2 antibody (Cat. #11453-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IHC, IP, WB,ELISA.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Conjugate: Unconjugated
Isotype: IgG
Vial size: 20ul, 150ul

FERMT2 protein structure - 11453-1-AP shown in red.

Submitted references Topology scaffolds-enhanced paracrine of BMSCs through mechanotransduction-related metabolism reprogramming for burn wounds healing.

Zhang Q, Li J, Jia J, He S, Mei A, Zhang Z, Cao Y, Zhang X, Zhang Y, Li Z, Luo G
Biomaterials 2026 Jan;324:123518

Talin-tensin3 interactions regulate fibrillar adhesion formation and tensin3 phase separation.

Li X, Konstantinou R, Meena VK, Notash S, Khalil K, Whalley T, Atherton P, Barsukov I, Zacharchenko T, Ballestrem C
The Journal of cell biology 2026 Jan 5;225(1)

Kindlin-2/Otub1/Slc7a11 Axis Improved Cardiac Ischemia Reperfusion Injury by Inhibiting Cardiomyocyte Ferroptosis.

Dong Y, Wu F, Liu K, Yue Y, Shen X, Qu Z, Yu S, Du W
Antioxidants & redox signaling 2025 Nov;43(13-15):727-744

FERMT2 drives anoikis resistance and peritoneal metastasis by enhancing extracellular matrix deposition in gastric cancer.

He C, Zhou Z, Yang Y, Zhu S, Wang H, Teng L
Gastric cancer : official journal of the International Gastric Cancer Association and the Japanese Gastric Cancer Association 2025 May;28(3):409-421

Activation of mannose receptor C type 1 in macrophages improves renal fibrosis through mediating fibronectin endocytosis.

Long L, Li M, Wang M, Liang B, Huang M, Yuan X, Wu X, Guo X, Li S, Liu Z, Liu W, Chen W, Wang W, Lyu Q, Li C
Life sciences 2025 Jun 15;371:123593

Mesenchymal stromal cells promote the formation of lung cancer organoids via Kindlin-2.

Sui Z, Wu X, Wang J, Tan S, Zhao C, Yu Z, Wu C, Wang X, Guo L
Stem cell research & therapy 2025 Jan 9;16(1):7

Multi-omics factor analysis identifies the Tensin 1-Fermitin family homologue 2-Fibronectin 1-Integrin signaling axis as a prognostic determinant in colorectal cancer.

Chen T, Yang Y, Shi J, Dong F, Xie L, Shan Y, Wang X
Molecular biomedicine 2025 Dec 17;6(1):141

Kindlin Regulates Mechanosensitive Activation and Adhesion Assembly of Integrin beta6.

Lee WN, Li J, Chen NP, Yu CH
Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2025 Aug;12(32):e01078

A flexible loop in the paxillin LIM3 domain mediates its direct binding to integrin β subunits.

Baade T, Michaelis M, Prestel A, Paone C, Klishin N, Herbinger M, Scheinost L, Nedielkov R, Hauck CR, Möller HM
PLoS biology 2024 Sep;22(9):e3002757

Kindlin-2 mediates Peyronie's disease through activation of TGF-β/Smad signaling pathway under the presence of TGF-β1.

Yang X, Wu J, Cai T, Shan J, Cui Y
Cellular signalling 2024 Sep;121:111286

TiO2 nanotube enhance osteogenesis through Kindlin-2/Integrin β1/YAP pathway-mediated mechanotransduction.

Deng Q, Yao Q, Wu A, Li J, Li Y, Tang L, Zeng H, Chen S, Guo T
Biomedical materials (Bristol, England) 2024 Sep 23;

The role of FERMT2 in the tumor microenvironment and immunotherapy in pan-cancer using comprehensive single-cell and bulk sequencing.

Wu GH, He C, Che G, Zhou Z, Chen BY, Wu HM, Chen JF, Zhu WP, Yang Y, Zhou Z, Teng LS, Wang HY
Heliyon 2024 May 15;10(9):e30505

Increased extracellular matrix stiffness regulates myofibroblast transformation through induction of autophagy-mediated Kindlin-2 cytoplasmic translocation.

Zhao Z, Han W, Huang G, He Y, Zuo X, Hong L
Experimental cell research 2024 Mar 15;436(2):113974

Engineered extracellular vesicles for targeted reprogramming of cancer-associated fibroblasts to potentiate therapy of pancreatic cancer.

Zhou P, Du X, Jia W, Feng K, Zhang Y
Signal transduction and targeted therapy 2024 Jun 24;9(1):151

Targeting miR-29 mitigates skeletal senescence and bolsters therapeutic potential of mesenchymal stromal cells.

Ding Z, Ma G, Zhou B, Cheng S, Tang W, Han Y, Chen L, Pang W, Chen Y, Yang D, Cao H
Cell reports. Medicine 2024 Aug 20;5(8):101665

Quantitative Proteomics Reveal the Mechanism of MiR-138-5p Suppressing Cervical Cancer via Targeting ZNF385A.

Peng Q, Deng Y, Li G, Li J, Zheng P, Xiong Q, Li J, Chen Y, Ge F
Journal of proteome research 2024 Aug 2;23(8):3659-3673

FERMT2 upregulation in CAFs enhances EMT of OSCC and M2 macrophage polarization.

Ma X, Zhao D, Liu S, Zuo J, Wang W, Wang F, Li Y, Ding Z, Wang J, Wang X
Oral diseases 2024 Apr;30(3):991-1003

Sarcoma Cells Secrete Hypoxia-Modified Collagen VI to Weaken the Lung Endothelial Barrier and Promote Metastasis.

Liu Y, Murazzi I, Fuller AM, Pan H, Irizarry-Negron VM, Devine A, Katti R, Skuli N, Ciotti GE, Pak K, Pack MA, Simon MC, Weber K, Cooper K, Eisinger-Mathason TSK
Cancer research 2024 Apr 1;84(7):977-993

Paxillin phase separation promotes focal adhesion assembly and integrin signaling.

Liang P, Wu Y, Zheng S, Zhang J, Yang S, Wang J, Ma S, Zhang M, Gu Z, Liu Q, Jiang W, Xing Q, Wang B
The Journal of cell biology 2024 Apr 1;223(4)

Kindlin-2 enhances c-Myc translation through association with DDX3X to promote pancreatic ductal adenocarcinoma progression.

Liu C, Jiang K, Ding Y, Yang A, Cai R, Bai P, Xiong M, Fu C, Quan M, Xiong Z, Deng Y, Tian R, Wu C, Sun Y
Theranostics 2023;13(13):4333-4355

An ULK1/2-PXN mechanotransduction pathway suppresses breast cancer cell migration.

Liang P, Zhang J, Wu Y, Zheng S, Xu Z, Yang S, Wang J, Ma S, Xiao L, Hu T, Jiang W, Huang C, Xing Q, Kundu M, Wang B
EMBO reports 2023 Nov 6;24(11):e56850

Distinct bidirectional regulation of LFA1 and α4β7 by Rap1 and integrin adaptors in T cells under shear flow.

Kamioka Y, Ueda Y, Kondo N, Tokuhiro K, Ikeda Y, Bergmeier W, Kinashi T
Cell reports 2023 Jun 27;42(6):112580

Cas phosphorylation regulates focal adhesion assembly.

Kumar S, Stainer A, Dubrulle J, Simpkins C, Cooper JA
eLife 2023 Jul 25;12

Molecular basis of astrocyte diversity and morphology across the CNS in health and disease.

Endo F, Kasai A, Soto JS, Yu X, Qu Z, Hashimoto H, Gradinaru V, Kawaguchi R, Khakh BS
Science (New York, N.Y.) 2022 Nov 4;378(6619):eadc9020

Kindlin-2 promotes Src-mediated tyrosine phosphorylation of androgen receptor and contributes to breast cancer progression.

Ma L, Tian Y, Qian T, Li W, Liu C, Chu B, Kong Q, Cai R, Bai P, Ma L, Deng Y, Tian R, Wu C, Sun Y
Cell death & disease 2022 May 20;13(5):482

Adherens junctions stimulate and spatially guide integrin activation and extracellular matrix deposition.

Hadjisavva R, Anastasiou O, Ioannou PS, Zheltkova M, Skourides PA
Cell reports 2022 Jul 19;40(3):111091

Construction of the optimization prognostic model based on differentially expressed immune genes of lung adenocarcinoma.

Zhai Y, Zhao B, Wang Y, Li L, Li J, Li X, Chang L, Chen Q, Liao Z
BMC cancer 2021 Mar 1;21(1):213

Kindlin-2 Acts as a Key Mediator of Lung Fibroblast Activation and Pulmonary Fibrosis Progression.

Zhang P, Wang J, Luo W, Yuan J, Cui C, Guo L, Wu C
American journal of respiratory cell and molecular biology 2021 Jul;65(1):54-69

Kindlin-2 promotes rear focal adhesion disassembly and directional persistence during cell migration.

Liu J, Liu Z, Chen K, Chen W, Fang X, Li M, Zhou X, Ding N, Lei H, Guo C, Qian T, Wang Y, Liu L, Chen Y, Zhao H, Sun Y, Deng Y, Wu C
Journal of cell science 2021 Jan 13;134(1)

Both Wnt signaling and epidermal stem cell-derived extracellular vesicles are involved in epidermal cell growth.

Leng L, Ma J, Lv L, Wang W, Gao D, Zhu Y, Wu Z
Stem cell research & therapy 2020 Sep 23;11(1):415

PINCH-1 regulates mitochondrial dynamics to promote proline synthesis and tumor growth.

Guo L, Cui C, Wang J, Yuan J, Yang Q, Zhang P, Su W, Bao R, Ran J, Wu C
Nature communications 2020 Oct 1;11(1):4913

Kindlin‑2 suppresses cervical cancer cell migration through AKT/mTOR‑mediated autophagy induction.

Wu G, Long Y, Lu Y, Feng Y, Yang X, Xu X, Yao D
Oncology reports 2020 Jul;44(1):69-76

Kindlin-2 links mechano-environment to proline synthesis and tumor growth.

Guo L, Cui C, Zhang K, Wang J, Wang Y, Lu Y, Chen K, Yuan J, Xiao G, Tang B, Sun Y, Wu C
Nature communications 2019 Feb 19;10(1):845

Kindlin-2 regulates hepatic stellate cells activation and liver fibrogenesis.

Yu J, Hu Y, Gao Y, Li Q, Zeng Z, Li Y, Chen H
Cell death discovery 2018;4:34

Kindlin-2 interacts with a highly conserved surface of ILK to regulate focal adhesion localization and cell spreading.

Kadry YA, Huet-Calderwood C, Simon B, Calderwood DA
Journal of cell science 2018 Oct 26;131(20)

Kindlin-2 Association with Rho GDP-Dissociation Inhibitor α Suppresses Rac1 Activation and Podocyte Injury.

Sun Y, Guo C, Ma P, Lai Y, Yang F, Cai J, Cheng Z, Zhang K, Liu Z, Tian Y, Sheng Y, Tian R, Deng Y, Xiao G, Wu C
Journal of the American Society of Nephrology : JASN 2017 Dec;28(12):3545-3562

Differences in binding to the ILK complex determines kindlin isoform adhesion localization and integrin activation.

Huet-Calderwood C, Brahme NN, Kumar N, Stiegler AL, Raghavan S, Boggon TJ, Calderwood DA
Journal of cell science 2014 Oct 1;127(Pt 19):4308-21

The integrin-linked kinase-PINCH-parvin complex supports integrin αIIbβ3 activation.

Honda S, Shirotani-Ikejima H, Tadokoro S, Tomiyama Y, Miyata T
PloS one 2013;8(12):e85498

Kindlin-2 expression in peritumoral stroma is associated with poor prognosis in pancreatic ductal adenocarcinoma.

Mahawithitwong P, Ohuchida K, Ikenaga N, Fujita H, Zhao M, Kozono S, Shindo K, Ohtsuka T, Mizumoto K, Tanaka M
Pancreas 2013 May;42(4):663-9

Kindlin binds migfilin tandem LIM domains and regulates migfilin focal adhesion localization and recruitment dynamics.

Brahme NN, Harburger DS, Kemp-O'Brien K, Stewart R, Raghavan S, Parsons M, Calderwood DA
The Journal of biological chemistry 2013 Dec 6;288(49):35604-16

Kindlin-3 mediates integrin αLβ2 outside-in signaling, and it interacts with scaffold protein receptor for activated-C kinase 1 (RACK1).

Feng C, Li YF, Yau YH, Lee HS, Tang XY, Xue ZH, Zhou YC, Lim WM, Cornvik TC, Ruedl C, Shochat SG, Tan SM
The Journal of biological chemistry 2012 Mar 30;287(14):10714-26

Comparative proteomic analysis of primary schwann cells and a spontaneously immortalized schwann cell line RSC 96: a comprehensive overview with a focus on cell adhesion and migration related proteins.

Ji Y, Shen M, Wang X, Zhang S, Yu S, Chen G, Gu X, Ding F
Journal of proteome research 2012 Jun 1;11(6):3186-98

Kindlin-2 expression in arsenite- and cadmium-transformed bladder cancer cell lines and in archival specimens of human bladder cancer.

Talaat S, Somji S, Toni C, Garrett SH, Zhou XD, Sens MA, Sens DA
Urology 2011 Jun;77(6):1507.e1-7

Kindlin-2 is an essential component of intercalated discs and is required for vertebrate cardiac structure and function.

Dowling JJ, Gibbs E, Russell M, Goldman D, Minarcik J, Golden JA, Feldman EL
Circulation research 2008 Feb 29;102(4):423-31

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Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: A549 cells were subjected to SDS PAGE followed by western blot with 11453-1-AP(FERMT2 antibody) at dilution of 1:500
Sample type: cell line

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunofluorescent analysis of HepG2 cells, using FERMT2 antibody 11453-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
Sample type: cell line

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: IP Result of anti-FERMT2 (IP:11453-1-AP, 3ug; Detection:11453-1-AP 1:1000) with A549 cells lysate 3300ug.
Sample type: cell line

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunohistochemical of paraffin-embedded human ovary tumor using 11453-1-AP(FERMT2 antibody) at dilution of 1:50 (under 10x lens)
Sample type: tissue