Antibody data
- Antibody Data
- Antigen structure
- References [28]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- 11453-1-AP - Provider product page
- Provider
- Proteintech Group
- Proper citation
- Proteintech Cat#11453-1-AP, RRID:AB_2262660
- Product name
- Kindlin 2 antibody
- Antibody type
- Polyclonal
- Description
- KD/KO validated Kindlin 2 antibody (Cat. #11453-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IHC, IP, WB,ELISA.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 20ul, 150ul
Submitted references Increased extracellular matrix stiffness regulates myofibroblast transformation through induction of autophagy-mediated Kindlin-2 cytoplasmic translocation.
FERMT2 upregulation in CAFs enhances EMT of OSCC and M2 macrophage polarization.
Sarcoma Cells Secrete Hypoxia-Modified Collagen VI to Weaken the Lung Endothelial Barrier and Promote Metastasis.
Kindlin-2 enhances c-Myc translation through association with DDX3X to promote pancreatic ductal adenocarcinoma progression.
An ULK1/2-PXN mechanotransduction pathway suppresses breast cancer cell migration.
Distinct bidirectional regulation of LFA1 and α4β7 by Rap1 and integrin adaptors in T cells under shear flow.
Cas phosphorylation regulates focal adhesion assembly.
Molecular basis of astrocyte diversity and morphology across the CNS in health and disease.
Kindlin-2 promotes Src-mediated tyrosine phosphorylation of androgen receptor and contributes to breast cancer progression.
Adherens junctions stimulate and spatially guide integrin activation and extracellular matrix deposition.
Construction of the optimization prognostic model based on differentially expressed immune genes of lung adenocarcinoma.
Kindlin-2 Acts as a Key Mediator of Lung Fibroblast Activation and Pulmonary Fibrosis Progression.
Kindlin-2 promotes rear focal adhesion disassembly and directional persistence during cell migration.
Both Wnt signaling and epidermal stem cell-derived extracellular vesicles are involved in epidermal cell growth.
PINCH-1 regulates mitochondrial dynamics to promote proline synthesis and tumor growth.
Kindlin‑2 suppresses cervical cancer cell migration through AKT/mTOR‑mediated autophagy induction.
Kindlin-2 links mechano-environment to proline synthesis and tumor growth.
Kindlin-2 regulates hepatic stellate cells activation and liver fibrogenesis.
Kindlin-2 interacts with a highly conserved surface of ILK to regulate focal adhesion localization and cell spreading.
Kindlin-2 Association with Rho GDP-Dissociation Inhibitor α Suppresses Rac1 Activation and Podocyte Injury.
Differences in binding to the ILK complex determines kindlin isoform adhesion localization and integrin activation.
The integrin-linked kinase-PINCH-parvin complex supports integrin αIIbβ3 activation.
Kindlin-2 expression in peritumoral stroma is associated with poor prognosis in pancreatic ductal adenocarcinoma.
Kindlin binds migfilin tandem LIM domains and regulates migfilin focal adhesion localization and recruitment dynamics.
Kindlin-3 mediates integrin αLβ2 outside-in signaling, and it interacts with scaffold protein receptor for activated-C kinase 1 (RACK1).
Comparative proteomic analysis of primary schwann cells and a spontaneously immortalized schwann cell line RSC 96: a comprehensive overview with a focus on cell adhesion and migration related proteins.
Kindlin-2 expression in arsenite- and cadmium-transformed bladder cancer cell lines and in archival specimens of human bladder cancer.
Kindlin-2 is an essential component of intercalated discs and is required for vertebrate cardiac structure and function.
Zhao Z, Han W, Huang G, He Y, Zuo X, Hong L
Experimental cell research 2024 Mar 15;436(2):113974
Experimental cell research 2024 Mar 15;436(2):113974
FERMT2 upregulation in CAFs enhances EMT of OSCC and M2 macrophage polarization.
Ma X, Zhao D, Liu S, Zuo J, Wang W, Wang F, Li Y, Ding Z, Wang J, Wang X
Oral diseases 2024 Apr;30(3):991-1003
Oral diseases 2024 Apr;30(3):991-1003
Sarcoma Cells Secrete Hypoxia-Modified Collagen VI to Weaken the Lung Endothelial Barrier and Promote Metastasis.
Liu Y, Murazzi I, Fuller AM, Pan H, Irizarry-Negron VM, Devine A, Katti R, Skuli N, Ciotti GE, Pak K, Pack MA, Simon MC, Weber K, Cooper K, Eisinger-Mathason TSK
Cancer research 2024 Apr 1;84(7):977-993
Cancer research 2024 Apr 1;84(7):977-993
Kindlin-2 enhances c-Myc translation through association with DDX3X to promote pancreatic ductal adenocarcinoma progression.
Liu C, Jiang K, Ding Y, Yang A, Cai R, Bai P, Xiong M, Fu C, Quan M, Xiong Z, Deng Y, Tian R, Wu C, Sun Y
Theranostics 2023;13(13):4333-4355
Theranostics 2023;13(13):4333-4355
An ULK1/2-PXN mechanotransduction pathway suppresses breast cancer cell migration.
Liang P, Zhang J, Wu Y, Zheng S, Xu Z, Yang S, Wang J, Ma S, Xiao L, Hu T, Jiang W, Huang C, Xing Q, Kundu M, Wang B
EMBO reports 2023 Nov 6;24(11):e56850
EMBO reports 2023 Nov 6;24(11):e56850
Distinct bidirectional regulation of LFA1 and α4β7 by Rap1 and integrin adaptors in T cells under shear flow.
Kamioka Y, Ueda Y, Kondo N, Tokuhiro K, Ikeda Y, Bergmeier W, Kinashi T
Cell reports 2023 Jun 27;42(6):112580
Cell reports 2023 Jun 27;42(6):112580
Cas phosphorylation regulates focal adhesion assembly.
Kumar S, Stainer A, Dubrulle J, Simpkins C, Cooper JA
eLife 2023 Jul 25;12
eLife 2023 Jul 25;12
Molecular basis of astrocyte diversity and morphology across the CNS in health and disease.
Endo F, Kasai A, Soto JS, Yu X, Qu Z, Hashimoto H, Gradinaru V, Kawaguchi R, Khakh BS
Science (New York, N.Y.) 2022 Nov 4;378(6619):eadc9020
Science (New York, N.Y.) 2022 Nov 4;378(6619):eadc9020
Kindlin-2 promotes Src-mediated tyrosine phosphorylation of androgen receptor and contributes to breast cancer progression.
Ma L, Tian Y, Qian T, Li W, Liu C, Chu B, Kong Q, Cai R, Bai P, Ma L, Deng Y, Tian R, Wu C, Sun Y
Cell death & disease 2022 May 20;13(5):482
Cell death & disease 2022 May 20;13(5):482
Adherens junctions stimulate and spatially guide integrin activation and extracellular matrix deposition.
Hadjisavva R, Anastasiou O, Ioannou PS, Zheltkova M, Skourides PA
Cell reports 2022 Jul 19;40(3):111091
Cell reports 2022 Jul 19;40(3):111091
Construction of the optimization prognostic model based on differentially expressed immune genes of lung adenocarcinoma.
Zhai Y, Zhao B, Wang Y, Li L, Li J, Li X, Chang L, Chen Q, Liao Z
BMC cancer 2021 Mar 1;21(1):213
BMC cancer 2021 Mar 1;21(1):213
Kindlin-2 Acts as a Key Mediator of Lung Fibroblast Activation and Pulmonary Fibrosis Progression.
Zhang P, Wang J, Luo W, Yuan J, Cui C, Guo L, Wu C
American journal of respiratory cell and molecular biology 2021 Jul;65(1):54-69
American journal of respiratory cell and molecular biology 2021 Jul;65(1):54-69
Kindlin-2 promotes rear focal adhesion disassembly and directional persistence during cell migration.
Liu J, Liu Z, Chen K, Chen W, Fang X, Li M, Zhou X, Ding N, Lei H, Guo C, Qian T, Wang Y, Liu L, Chen Y, Zhao H, Sun Y, Deng Y, Wu C
Journal of cell science 2021 Jan 13;134(1)
Journal of cell science 2021 Jan 13;134(1)
Both Wnt signaling and epidermal stem cell-derived extracellular vesicles are involved in epidermal cell growth.
Leng L, Ma J, Lv L, Wang W, Gao D, Zhu Y, Wu Z
Stem cell research & therapy 2020 Sep 23;11(1):415
Stem cell research & therapy 2020 Sep 23;11(1):415
PINCH-1 regulates mitochondrial dynamics to promote proline synthesis and tumor growth.
Guo L, Cui C, Wang J, Yuan J, Yang Q, Zhang P, Su W, Bao R, Ran J, Wu C
Nature communications 2020 Oct 1;11(1):4913
Nature communications 2020 Oct 1;11(1):4913
Kindlin‑2 suppresses cervical cancer cell migration through AKT/mTOR‑mediated autophagy induction.
Wu G, Long Y, Lu Y, Feng Y, Yang X, Xu X, Yao D
Oncology reports 2020 Jul;44(1):69-76
Oncology reports 2020 Jul;44(1):69-76
Kindlin-2 links mechano-environment to proline synthesis and tumor growth.
Guo L, Cui C, Zhang K, Wang J, Wang Y, Lu Y, Chen K, Yuan J, Xiao G, Tang B, Sun Y, Wu C
Nature communications 2019 Feb 19;10(1):845
Nature communications 2019 Feb 19;10(1):845
Kindlin-2 regulates hepatic stellate cells activation and liver fibrogenesis.
Yu J, Hu Y, Gao Y, Li Q, Zeng Z, Li Y, Chen H
Cell death discovery 2018;4:34
Cell death discovery 2018;4:34
Kindlin-2 interacts with a highly conserved surface of ILK to regulate focal adhesion localization and cell spreading.
Kadry YA, Huet-Calderwood C, Simon B, Calderwood DA
Journal of cell science 2018 Oct 26;131(20)
Journal of cell science 2018 Oct 26;131(20)
Kindlin-2 Association with Rho GDP-Dissociation Inhibitor α Suppresses Rac1 Activation and Podocyte Injury.
Sun Y, Guo C, Ma P, Lai Y, Yang F, Cai J, Cheng Z, Zhang K, Liu Z, Tian Y, Sheng Y, Tian R, Deng Y, Xiao G, Wu C
Journal of the American Society of Nephrology : JASN 2017 Dec;28(12):3545-3562
Journal of the American Society of Nephrology : JASN 2017 Dec;28(12):3545-3562
Differences in binding to the ILK complex determines kindlin isoform adhesion localization and integrin activation.
Huet-Calderwood C, Brahme NN, Kumar N, Stiegler AL, Raghavan S, Boggon TJ, Calderwood DA
Journal of cell science 2014 Oct 1;127(Pt 19):4308-21
Journal of cell science 2014 Oct 1;127(Pt 19):4308-21
The integrin-linked kinase-PINCH-parvin complex supports integrin αIIbβ3 activation.
Honda S, Shirotani-Ikejima H, Tadokoro S, Tomiyama Y, Miyata T
PloS one 2013;8(12):e85498
PloS one 2013;8(12):e85498
Kindlin-2 expression in peritumoral stroma is associated with poor prognosis in pancreatic ductal adenocarcinoma.
Mahawithitwong P, Ohuchida K, Ikenaga N, Fujita H, Zhao M, Kozono S, Shindo K, Ohtsuka T, Mizumoto K, Tanaka M
Pancreas 2013 May;42(4):663-9
Pancreas 2013 May;42(4):663-9
Kindlin binds migfilin tandem LIM domains and regulates migfilin focal adhesion localization and recruitment dynamics.
Brahme NN, Harburger DS, Kemp-O'Brien K, Stewart R, Raghavan S, Parsons M, Calderwood DA
The Journal of biological chemistry 2013 Dec 6;288(49):35604-16
The Journal of biological chemistry 2013 Dec 6;288(49):35604-16
Kindlin-3 mediates integrin αLβ2 outside-in signaling, and it interacts with scaffold protein receptor for activated-C kinase 1 (RACK1).
Feng C, Li YF, Yau YH, Lee HS, Tang XY, Xue ZH, Zhou YC, Lim WM, Cornvik TC, Ruedl C, Shochat SG, Tan SM
The Journal of biological chemistry 2012 Mar 30;287(14):10714-26
The Journal of biological chemistry 2012 Mar 30;287(14):10714-26
Comparative proteomic analysis of primary schwann cells and a spontaneously immortalized schwann cell line RSC 96: a comprehensive overview with a focus on cell adhesion and migration related proteins.
Ji Y, Shen M, Wang X, Zhang S, Yu S, Chen G, Gu X, Ding F
Journal of proteome research 2012 Jun 1;11(6):3186-98
Journal of proteome research 2012 Jun 1;11(6):3186-98
Kindlin-2 expression in arsenite- and cadmium-transformed bladder cancer cell lines and in archival specimens of human bladder cancer.
Talaat S, Somji S, Toni C, Garrett SH, Zhou XD, Sens MA, Sens DA
Urology 2011 Jun;77(6):1507.e1-7
Urology 2011 Jun;77(6):1507.e1-7
Kindlin-2 is an essential component of intercalated discs and is required for vertebrate cardiac structure and function.
Dowling JJ, Gibbs E, Russell M, Goldman D, Minarcik J, Golden JA, Feldman EL
Circulation research 2008 Feb 29;102(4):423-31
Circulation research 2008 Feb 29;102(4):423-31
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Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- A549 cells were subjected to SDS PAGE followed by western blot with 11453-1-AP(FERMT2 antibody) at dilution of 1:500
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of HepG2 cells, using FERMT2 antibody 11453-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- IP Result of anti-FERMT2 (IP:11453-1-AP, 3ug; Detection:11453-1-AP 1:1000) with A549 cells lysate 3300ug.
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunohistochemical of paraffin-embedded human ovary tumor using 11453-1-AP(FERMT2 antibody) at dilution of 1:50 (under 10x lens)
- Sample type
- tissue