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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [2]
- Immunoprecipitation [1]
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Validation data
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- Product number
- TA500509 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA500509, RRID:AB_11125982
- Product name
- Anti-FERMT2 mouse monoclonal antibody, clone OTI22E7 (formerly 22E7)
- Antibody type
- Monoclonal
- Description
- Anti-FERMT2 mouse monoclonal antibody, clone OTI22E7 (formerly 22E7)
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- FERMT2
- Isotype
- IgG
- Antibody clone number
- OTI22E7
- Vial size
- 100 µl
- Concentration
- 1.00mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FERMT2 (RC205727, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FERMT2.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (10ug) from 4 different cell lines by using anti-FERMT2 monoclonal antibody.(1:200)
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunofluorescent staining of HeLa cells using anti-FERMT2 mouse monoclonal antibody (TA500509).
- Validation comment
- IF
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Anti-FERMT2 mouse monoclonal antibody (TA500509) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY FERMT2(RC205727).
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunoprecipitation of FERMT2 by using TrueMab monoclonal anti-FERMT2 antibodies (Negative control: IP without adding anti-FERMT2 antibody.). For each experiment, 500ul of DDK tagged FERMT2 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-FERMT2 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.
- Validation comment
- IP
