MAB7777

ZG16B antibody from Novus Biologicals

HRPE773, JCLN2, PRO1567

Western blot

Supportive data in Antibodypedia

Immunohistochemistry

Supportive data in Antibodypedia

Provider product page for MAB7777

Antibody Data

Product number

MAB7777

Provider

Novus Biologicals

Product name

Mouse Monoclonal PAUF/ZG16B Antibody

Provider product page

Antibody type

Monoclonal

Description

Protein A or G purified from hybridoma culture supernatant. Detects human PAUF/ZG16B in direct ELISAs and Western blots.

Reactivity

Human

Host

Mouse

Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

References

Elevated expression of pancreatic adenocarcinoma upregulated factor (PAUF) is associated with poor prognosis and chemoresistance in epithelial ovarian cancer.
Choi CH, Kang TH, Song JS, Kim YS, Chung EJ, Ylaya K, Kim S, Koh SS, Chung JY, Kim JH, Hewitt SM
Scientific reports 2018 Aug 15;8(1):12161

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Detection of Human PAUF/ZG16B by Western Blot. Detection of Human PAUF/ZG16B by Western Blot.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Detection of Human PAUF/ZG16B by Western Blot. Western blot shows lysates of human placenta tissue and human prostate tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human PAUF/ZG16B Monoclonal Antibody (Catalog # MAB7777) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for PAUF/ZG16B at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

PAUF/ZG16B in Human Pancreas Cancer Tissue. PAUF/ZG16B in Human Pancreas Cancer Tissue.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

PAUF/ZG16B in Human Pancreas Cancer Tissue. PAUF/ZG16B was detected in immersion fixed paraffin-embedded sections of human pancreas cancer tissue using Mouse Anti-Human PAUF/ZG16B Monoclonal Antibody (Catalog # MAB7777) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and plasma membranes of cancer cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.