MA5-17099

antibody from Invitrogen Antibodies

Targeting: ILK

ELISA

Antibody data

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Validation data

Product number: MA5-17099 - Provider product page
Provider: Invitrogen Antibodies
Product name: ILK Monoclonal Antibody (3A9)
Antibody type: Monoclonal
Antigen: Purifed from natural sources
Description: MA5-17099 targets ILK in indirect ELISA, FACS, IHC, and WB applications and shows reactivity with Human, Mouse, and Non-human primate samples. The MA5-17099 immunogen is purified recombinant fragment of human ILK (amino acids: 97-244) expressed in E. Coli. MA5-17099 detects ILK which has a predicted molecular weight of approximately 51.4kDa.
Reactivity: Human, Mouse, Rat
Host: Mouse
Isotype: IgG
Antibody clone number: 3A9
Vial size: 100 µL
Concentration: Conc. Not Determined
Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

ILK protein structure - MA5-17099 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of ILK using a ILK monoclonal antibody (Product # MA5-17099) against a human ILK recombinant protein.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of ILK using a ILK monoclonal antibody (Product # MA5-17099) against a human ILK recombinant protein.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of ILK using ILK monoclonal antibody (Product # MA5-17099) in Jurkat (1), NIH3T3 (2), HeLa (3), PC-12 (4), C6 (5), COS-7 (6), Raji (7), K562 (8) and MCF-7 (9) cell lysate.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot was performed using Anti-ILK Monoclonal Antibody (3A9) (Product # MA5-17099) and a 51 kDa band corresponding to Integrin-linked protein kinase was observed across cell lines and tissues. Whole cell extracts (30 µg lysate) of MDA-MB-231 (Lane 1), HeLa (Lane 2), Daudi (Lane 3), T-47D (Lane 4), PC-3 (Lane 5), LNCaP (Lane 6), NIH/3T3 (Lane 7), Mouse Lung (Lane 8) and Rat Lung (Lane 9) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:8000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).