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Western blot
Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [4]
- ELISA [1]
- Immunocytochemistry [1]
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- Product number
- H00003611-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00003611-M01, RRID:AB_539851
- Product name
- Mouse Monoclonal ILK Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. ILK - integrin-linked kinase
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: ILK Antibody (4F10) [H00003611-M01] - Analysis of ILK over-expressed 293 cell line, cotransfected with ILK Validated Chimera RNAi ( Cat # H00003611-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with ILK monoclonal antibody (M01) clone 4F10 (Cat # H00003611-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: ILK Antibody (4F10) [H00003611-M01] - Analysis of ILK expression in transfected 293T cell line by ILK monoclonal antibody (M01), clone 4F10.Lane 1: ILK transfected lysate(51.4 KDa).Lane 2: Non-transfected lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: ILK Antibody (4F10) [H00003611-M01] - ILK monoclonal antibody (M01), clone 4F10. Analysis of ILK expression in HepG2.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: ILK Antibody (4F10) [H00003611-M01] - ILK monoclonal antibody (M01), clone 4F10 Analysis of ILK expression in HeLa.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Sandwich ELISA: ILK Antibody (4F10) [H00003611-M01] - Detection limit for recombinant GST tagged ILK is approximately 3ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: ILK Antibody (4F10) [H00003611-M01] - Analysis of monoclonal antibody to ILK on Jurkat cell . Antibody concentration 10 ug/ml.
