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ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [3]
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- Product number
- RQ6426 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- Integrin linked protein kinase Antibody / ILK
- Antibody type
- Polyclonal
- Description
- This highly specific Integrin linked protein kinase antibody is suitable for use in Western blot/Immunofluorescence/Immunohistochemistry/Direct ELISA applications with human, mouse and rat samples.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 100 ug
- Concentration
- 0.5mg/ml if reconstituted with 0.2ml sterile DI water
- Storage
- After reconstitution, the Integrin linked protein kinase antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human 1) MCF7, 2) HeLa, 3) U-87 MG, 4) A431, 5) U-2 OS, 6) PC-3, 7) rat heart, 8) rat kidney, 9) rat skeletal muscle, 10) mouse heart, 11) mouse kidney, 12) mouse skeletal muscle and 13) mouse C2C12 lysate with Integrin linked protein kinase antibody. Expected molecular weight: 51-59 kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Immunofluorescent staining of FFPE human A431 cells with Integrin linked protein kinase antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE bladder epithelial carcinoma tissue with Integrin linked protein kinase antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE human breast cancer tissue with Integrin linked protein kinase antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE human laryngeal squamous cell carcinoma tissue with Integrin linked protein kinase antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
