Explore
Validate
Learn
Western blot
Other assayAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Other assay [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA5-27484 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ILK Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: HeLa, A431, Neuro 2A, C8D30, NIH-3T3, Raw264.7, C2C12, PC-12. Predicted reactivity: Mouse (99%), Rat (99%), Zebrafish (81%), Xenopus laevis (84%), Chicken (86%), Sheep (97%), Rhesus Monkey (100%), Bovine (98%), Guinea pig (99%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.8 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Enhancement of aberrantly modified integrin‑mediated cell motility in multicellular tumor spheroids.
Kim B, Im NR, Yang TD, Kim J, Jung KY, Kim TH, Baek SK
International journal of oncology 2020 Jun;56(6):1490-1498
International journal of oncology 2020 Jun;56(6):1490-1498
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- ILK Polyclonal Antibody immunoprecipitates ILK protein in IP experiments. IP samples: HeLa whole cell extract. A. 30 µg HeLa whole cell extract. B. Control with 4 µg of preimmune Rabbit IgG. C. Immunoprecipitation of ILK protein by 4 µg ILK Polyclonal Antibody (Product # PA5-27484). 10 % SDS-PAGE. The immunoprecipitated ILK protein was detected by ILK Polyclonal Antibody (Product # PA5-27484) diluted at 1:500.
