H00064946-M01

antibody from Abnova Corporation

Targeting: CENPH

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Antibody Data

Product number

H00064946-M01

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00064946-M01, RRID:AB_1204138

Product name

CENPH monoclonal antibody (M01), clone 1F7

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a full-length recombinant CENPH.

Antigen sequence

MEEQPQMQDADEPADSGGEGRAGGPPQVAGAQAAC
SEDRMTLLLRLRAQTKQQLLEYKSMVDASEEKTPE
QIMQEKQIEAKIEDLENEIEEVKVAFEIKKLALDR
MRLSTALKKNLEKISRQSSVLMDNMKHLLELNKLI
MKSQQESWDLEEKLLDIRKKRLQLKQASESKLLEI
QTEKNKQKIDLDSMENSERIKIIRQNLQMEIKITT
VIQHVFQNLILGSKVNWAEDPALKEIVLQLEKNVD
MM


Isotype

IgG

Antibody clone number

1F7

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of CENPH expression in transfected 293T cell line by CENPH monoclonal antibody (M01), clone 1F7.Lane 1: CENPH transfected lysate(28.5 KDa).Lane 2: Non-transfected lysate.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged CENPH is 0.03 ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol