Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Immunohistochemistry [1]
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Validation data
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- Product number
- MAB1039 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal 2B4/CD244/SLAMF4 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human 2B4/CD244/SLAMF4 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant mouse 2B4/CD244/SLAMF4 is observed.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 500 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Shear flow-dependent integration of apical and subendothelial chemokines in T-cell transmigration: implications for locomotion and the multistep paradigm.
Schreiber TH, Shinder V, Cain DW, Alon R, Sackstein R
Blood 2007 Feb 15;109(4):1381-6
Blood 2007 Feb 15;109(4):1381-6
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- 2B4/CD244/SLAMF4 in Human Spleen. 2B4/CD244/SLAMF4 was detected in immersion fixed paraffin-embedded sections of human spleen using Mouse Anti-Human 2B4/CD244/SLAMF4 Monoclonal Antibody (Catalog # MAB1039) at 1 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to splenocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.