Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- PA5-47050 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD244 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Reconstitute at 0.2 mg/mL in sterile PBS.
- Concentration
- 0.2 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of CD244 in NK‚RL human natural killer lymphocytic leukemia cell line, NK‚92 human natural killer lymphoma cell line, and Daudi human Burkitts lymphoma cell line (negative control). Samples were incubated in CD244 polyclonal antibody (Product # PA5-47050) using a dilution of 0.5 µg/mL followed by a HRP-conjugated Anti-Goat IgG secondary antibody. A specific band was detected for 2B4/CD244/SLAMF4 at approximately 65 kDa (as indicated). GAPDH is shown as a loading control. This experiment was conducted under reducing conditions.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of CD244 in immersion fixed paraffin-embedded sections of human spleen. Samples were incubated with CD244 polyclonal antibody (Product # PA5-47050) using a dilution of 1 µg/mL for 1 hour at room temperature followed by Anti-Goat IgG VisUCyte™ HRP Polymer Antibody. Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to splenocytes.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of CD244 in Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human NCAM‚1/CD56 APC‚conjugated Monoclonal Antibody. Samples were incubated in either (A) CD244 polyclonal antibody (Product # PA5-47050) or (B) Normal Goat IgG Control followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody.