Antibody data
- Antibody Data
- Antigen structure
- References [6]
- Comments [0]
- Validations
- Flow cytometry [1]
- Other assay [1]
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Validation data
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- Product number
- 11-5837-42 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD244 Monoclonal Antibody (eBioDM244), FITC, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The eBioDM244 monoclonal antibody reacts with human CD244 (2B4, p38). In human, CD244 is a 38 kDa protein expressed on NK cells, a subset of CD8+ T cells, gamma delta T cells, monocytes, basophils and eosinophils. Binding of the CD244 ligand, CD48, results in NK cell activation, unlike mouse CD244, which is an inhibitory receptor. Applications Reported: This eBioDM244 antibody has been reported for use in flow cytometric analysis. Applications Tested: This eBioDM244 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Green dye
- Isotype
- IgG
- Antibody clone number
- eBioDM244
- Vial size
- 100 Tests
- Concentration
- 5 µL/Test
- Storage
- 4° C, store in dark, DO NOT FREEZE!
Submitted references A transcriptionally and functionally distinct PD-1(+) CD8(+) T cell pool with predictive potential in non-small-cell lung cancer treated with PD-1 blockade.
TIGIT expressing CD4+T cells represent a tumor-supportive T cell subset in chronic lymphocytic leukemia.
Prolongation of overall survival in advanced lung adenocarcinoma patients with the XAGE1 (GAGED2a) antibody.
Early expression of triggering receptors and regulatory role of 2B4 in human natural killer cell precursors undergoing in vitro differentiation.
2B4-mediated activation of human natural killer cells.
Molecular characterization of a novel human natural killer cell receptor homologous to mouse 2B4.
Thommen DS, Koelzer VH, Herzig P, Roller A, Trefny M, Dimeloe S, Kiialainen A, Hanhart J, Schill C, Hess C, Savic Prince S, Wiese M, Lardinois D, Ho PC, Klein C, Karanikas V, Mertz KD, Schumacher TN, Zippelius A
Nature medicine 2018 Jul;24(7):994-1004
Nature medicine 2018 Jul;24(7):994-1004
TIGIT expressing CD4+T cells represent a tumor-supportive T cell subset in chronic lymphocytic leukemia.
Catakovic K, Gassner FJ, Ratswohl C, Zaborsky N, Rebhandl S, Schubert M, Steiner M, Gutjahr JC, Pleyer L, Egle A, Hartmann TN, Greil R, Geisberger R
Oncoimmunology 2017;7(1):e1371399
Oncoimmunology 2017;7(1):e1371399
Prolongation of overall survival in advanced lung adenocarcinoma patients with the XAGE1 (GAGED2a) antibody.
Ohue Y, Kurose K, Mizote Y, Matsumoto H, Nishio Y, Isobe M, Fukuda M, Uenaka A, Oka M, Nakayama E
Clinical cancer research : an official journal of the American Association for Cancer Research 2014 Oct 1;20(19):5052-63
Clinical cancer research : an official journal of the American Association for Cancer Research 2014 Oct 1;20(19):5052-63
Early expression of triggering receptors and regulatory role of 2B4 in human natural killer cell precursors undergoing in vitro differentiation.
Sivori S, Falco M, Marcenaro E, Parolini S, Biassoni R, Bottino C, Moretta L, Moretta A
Proceedings of the National Academy of Sciences of the United States of America 2002 Apr 2;99(7):4526-31
Proceedings of the National Academy of Sciences of the United States of America 2002 Apr 2;99(7):4526-31
2B4-mediated activation of human natural killer cells.
Tangye SG, Cherwinski H, Lanier LL, Phillips JH
Molecular immunology 2000 Jun;37(9):493-501
Molecular immunology 2000 Jun;37(9):493-501
Molecular characterization of a novel human natural killer cell receptor homologous to mouse 2B4.
Boles KS, Nakajima H, Colonna M, Chuang SS, Stepp SE, Bennett M, Kumar V, Mathew PA
Tissue antigens 1999 Jul;54(1):27-34
Tissue antigens 1999 Jul;54(1):27-34
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Staining of normal human peripheral blood cells with Anti-Human CD56 (NCAM) PE (Product # 12-0567-42) and Mouse IgG1 kappa Isotype Control FITC (Product # 11-4714-42) (left) or Anti-Human CD244 FITC (right). Total viable cells were used for analysis.
- Conjugate
- Green dye
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Conjugate
- Green dye