Antibody data
- Antibody Data
- Antigen structure
- References [7]
- Comments [0]
- Validations
- Flow cytometry [1]
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- Product number
- 13-5838-82 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD244 Monoclonal Antibody (eBioC1.7 (C1.7)), Biotin, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The eBioC1.7 monoclonal antibody reacts with human CD244 (2B4, p38). In human, CD244 is a 38 kDa protein expressed on NK cells, a subset of CD8+ T cells, gammadelta T cells, monocytes, basophils and eosinophils. Binding of the CD244 ligand, CD48, results in NK cell activation, unlike mouse CD244, which is an inhibitory receptor. For CD244 expressed on NK cells, binding of CD48 results in enhanced NK cell cytotoxicity and secretion of IFN-gamma. Recently, it was demonstrated that binding of the C1.7 monoclonal antibody and CD48 involve the same residue in the V domain of human CD244, which explains the ability of C1.7 binding to induce activation of NK cells. Binding of C1.7 to CD244 leads to tyrosine phosphorylation and recruitment of the adaptor molecule SAP (SLAM-associated protein). Patients with X-linked lymphoproliferative disorder (XLPD) have a mutation in SAP which renders it unable to bind to phosphorylated CD244.
- Conjugate
- Biotin
- Antibody clone number
- eBioC1.7 (C1.7)
- Concentration
- 0.5 mg/mL
Submitted references Targeting Ewing sarcoma with activated and GD2-specific chimeric antigen receptor-engineered human NK cells induces upregulation of immune-inhibitory HLA-G.
Inhibition of the B7-H3 immune checkpoint limits tumor growth by enhancing cytotoxic lymphocyte function.
Clonal expansion of CD4(+) cytotoxic T lymphocytes in patients with IgG4-related disease.
Human cytomegalovirus drives epigenetic imprinting of the IFNG locus in NKG2Chi natural killer cells.
Inhibitory signaling blocks activating receptor clustering and induces cytoskeletal retraction in natural killer cells.
Basophils play a critical role in the development of IgE-mediated chronic allergic inflammation independently of T cells and mast cells.
Identification of a novel signal transduction surface molecule on human cytotoxic lymphocytes.
Kailayangiri S, Altvater B, Spurny C, Jamitzky S, Schelhaas S, Jacobs AH, Wiek C, Roellecke K, Hanenberg H, Hartmann W, Wiendl H, Pankratz S, Meltzer J, Farwick N, Greune L, Fluegge M, Rossig C
Oncoimmunology 2017;6(1):e1250050
Oncoimmunology 2017;6(1):e1250050
Inhibition of the B7-H3 immune checkpoint limits tumor growth by enhancing cytotoxic lymphocyte function.
Lee YH, Martin-Orozco N, Zheng P, Li J, Zhang P, Tan H, Park HJ, Jeong M, Chang SH, Kim BS, Xiong W, Zang W, Guo L, Liu Y, Dong ZJ, Overwijk WW, Hwu P, Yi Q, Kwak L, Yang Z, Mak TW, Li W, Radvanyi LG, Ni L, Liu D, Dong C
Cell research 2017 Aug;27(8):1034-1045
Cell research 2017 Aug;27(8):1034-1045
Clonal expansion of CD4(+) cytotoxic T lymphocytes in patients with IgG4-related disease.
Mattoo H, Mahajan VS, Maehara T, Deshpande V, Della-Torre E, Wallace ZS, Kulikova M, Drijvers JM, Daccache J, Carruthers MN, Castelino FV, Stone JR, Stone JH, Pillai S
The Journal of allergy and clinical immunology 2016 Sep;138(3):825-838
The Journal of allergy and clinical immunology 2016 Sep;138(3):825-838
Human cytomegalovirus drives epigenetic imprinting of the IFNG locus in NKG2Chi natural killer cells.
Luetke-Eversloh M, Hammer Q, Durek P, Nordström K, Gasparoni G, Pink M, Hamann A, Walter J, Chang HD, Dong J, Romagnani C
PLoS pathogens 2014 Oct;10(10):e1004441
PLoS pathogens 2014 Oct;10(10):e1004441
Inhibitory signaling blocks activating receptor clustering and induces cytoskeletal retraction in natural killer cells.
Abeyweera TP, Merino E, Huse M
The Journal of cell biology 2011 Feb 21;192(4):675-90
The Journal of cell biology 2011 Feb 21;192(4):675-90
Basophils play a critical role in the development of IgE-mediated chronic allergic inflammation independently of T cells and mast cells.
Mukai K, Matsuoka K, Taya C, Suzuki H, Yokozeki H, Nishioka K, Hirokawa K, Etori M, Yamashita M, Kubota T, Minegishi Y, Yonekawa H, Karasuyama H
Immunity 2005 Aug;23(2):191-202
Immunity 2005 Aug;23(2):191-202
Identification of a novel signal transduction surface molecule on human cytotoxic lymphocytes.
Valiante NM, Trinchieri G
The Journal of experimental medicine 1993 Oct 1;178(4):1397-406
The Journal of experimental medicine 1993 Oct 1;178(4):1397-406
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Staining of normal human peripheral blood cells with Anti-Human CD8 APC (cat. 17-0086) and 0.125 µg of Mouse IgG1 kappa Isotype Control Biotin (cat. 13-4714) (left) or 0.125 µg of Anti-Human CD244 Biotin (right) followed by Streptavidin PE (cat. 12-4317). Cells in the lymphocyte gate were used for analysis.
- Conjugate
- Biotin