Antibody data
- Antibody Data
- Antigen structure
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- Validations
- Flow cytometry [2]
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- Product number
- 62-5838-42 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD244 Monoclonal Antibody (eBioC1.7 (C1.7)), Super Bright™ 436, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The eBioC1.7 monoclonal antibody reacts with human CD244 (2B4, p38). In human, CD244 is a 38 kDa protein expressed on NK cells, a subset of CD8+ T cells, gammadelta T cells, monocytes, basophils and eosinophils. Binding of the CD244 ligand, CD48, results in NK cell activation, unlike mouse CD244, which is an inhibitory receptor. For CD244 expressed on NK cells, binding of CD48 results in enhanced NK cell cytotoxicity and secretion of IFN-gamma. Recently, it was demonstrated that binding of the C1.7 monoclonal antibody and CD48 involve the same residue in the V domain of human CD244, which explains the ability of C1.7 binding to induce activation of NK cells. Binding of C1.7 to CD244 leads to tyrosine phosphorylation and recruitment of the adaptor molecule SAP (SLAM-associated protein). Patients with X-linked lymphoproliferative disorder (XLPD) have a mutation in SAP which renders it unable to bind to phosphorylated CD244. Applications Reported: This eBioC1.7 (C1.7) antibody has been reported for use in flow cytometric analysis. Applications Tested: This eBioC1.7 (C1.7) antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Super Bright 436 can be excited with the violet laser line (405 nm) and emits at 436 nm. We recommend using a 450/50 bandpass filter, or equivalent. Please make sure that your instrument is capable of detecting this fluorochrome. When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer for more information. Excitation: 405 nm; Emission: 436 nm; Laser: Violet Laser Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- eBioC1.7 (C1.7)
- Vial size
- 100 Tests
- Concentration
- 5 µL/Test
- Storage
- 4° C, store in dark, DO NOT FREEZE!
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Normal human peripheral blood cells were stained with CD8a Monoclonal Antibody, FITC (Product # 11-0087-42) and Mouse IgG1 kappa Isotype Control, Super Bright 436 (Product # 62-4714-82) (left) or CD244 Monoclonal Antibody, Super Bright 436 (right). Cells in the lymphocyte gate were used for analysis.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Normal human peripheral blood cells were stained with CD8a Monoclonal Antibody, FITC (Product # 11-0087-42) and Mouse IgG1 kappa Isotype Control, Super Bright 436 (Product # 62-4714-82) (left) or CD244 Monoclonal Antibody, Super Bright 436 (right). Cells in the lymphocyte gate were used for analysis.