16-2449-81

antibody from Invitrogen Antibodies

Targeting: CD244 2B4, NAIL, NKR2B4, Nmrk, SLAMF4

Flow cytometry (Supportive data in Antibodypedia)

Other assay (Recommended by provider)

Antibody Data

Product number

16-2449-81

Provider

Invitrogen Antibodies

Product name

CD244 Monoclonal Antibody (eBioPP35 (PP35)), Functional Grade, eBioscience™

Antibody type

Monoclonal

Antigen

Other

Description

Description: The eBioPP35 monoclonal antibody reacts with human CD244, also known as 2B4, p38, NK-cell activation-inducing ligand, signaling lymphocyte activation molecule family member 4, and natural killer cell receptor 2B4. Human CD244 is a 38 kD protein expressed on NK cells, a subset of CD8+ T cells, gammadelta T cells, monocytes, basophils, mast cells, and eosinophils. Binding of CD244 to its ligand, CD48, results in NK cell activation. Costaining experiments with eBioC1.7 suggest that both antibodies recognize different epitopes. Applications Tested: This eBioPP35 (PP35) antibody has been tested by flow cytometric analysis of human peripheral blood cells. Storage and handling: Use in a sterile environment. Filtration: 0.2 µm post-manufacturing filtered. Purity: Greater than 90%, as determined by SDS-PAGE. Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay. Aggregation: Less than 10%, as determined by HPLC.

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

eBioPP35 (PP35)

Vial size

50 µg

Concentration

1 mg/mL

Storage

4° C

References

Submitted references

FOXP3 protects conventional human T cells from premature restimulation-induced cell death.

Voss K, Lake C, Luthers CR, Lott NM, Dorjbal B, Arjunaraja S, Bauman BM, Soltis AR, Sukumar G, Dalgard CL, Snow AL
Cellular & molecular immunology 2021 Jan;18(1):194-205

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Targeting LAG-3 and PD-1 to Enhance T Cell Activation by Antigen-Presenting Cells.

Lichtenegger FS, Rothe M, Schnorfeil FM, Deiser K, Krupka C, Augsberger C, Schlüter M, Neitz J, Subklewe M
Frontiers in immunology 2018;9:385

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Genetic predisposition to hemophagocytic lymphohistiocytosis: Report on 500 patients from the Italian registry.

Cetica V, Sieni E, Pende D, Danesino C, De Fusco C, Locatelli F, Micalizzi C, Putti MC, Biondi A, Fagioli F, Moretta L, Griffiths GM, Luzzatto L, Aricò M
The Journal of allergy and clinical immunology 2016 Jan;137(1):188-196.e4

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Diagnosing XLP1 in patients with hemophagocytic lymphohistiocytosis.

Meazza R, Tuberosa C, Cetica V, Falco M, Parolini S, Grieve S, Griffiths GM, Sieni E, Marcenaro S, Micalizzi C, Montin D, Fagioli F, Moretta A, Mingari MC, Moretta L, Notarangelo LD, Bottino C, Aricò M, Pende D
The Journal of allergy and clinical immunology 2014 Dec;134(6):1381-1387.e7

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Involvement of CD244 in regulating CD4+ T cell immunity in patients with active tuberculosis.

Yang B, Wang X, Jiang J, Cheng X
PloS one 2013;8(4):e63261

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Dual function of the NK cell receptor 2B4 (CD244) in the regulation of HCV-specific CD8+ T cells.

Schlaphoff V, Lunemann S, Suneetha PV, Jaroszewicz J, Grabowski J, Dietz J, Helfritz F, Bektas H, Sarrazin C, Manns MP, Cornberg M, Wedemeyer H
PLoS pathogens 2011 May;7(5):e1002045

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Staining of normal human peripheral blood cells with Anti-Human CD8a APC (Product # 17-0088-42) and 0.25 µg of Mouse IgG1 K Isotype Control Functional Grade Purified (Product # 16-4714-82) (left) or 0.25 µg of Anti-Human CD244 Functional Grade Purified (right). Cells in the lymphocyte gate were used for analysis.