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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
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Validation data
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- Product number
- GTX116932 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX116932, RRID:AB_11169569
- Product name
- SETDB2 antibody [N1N2], N-term
- Antibody type
- Polyclonal
- Reactivity
- Human
- Host
- Rabbit
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ug of whole cell lysate) A: SK-N-SH 7.5% SDS PAGE GTX116932 diluted at 1:1000
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- SETDB2 antibody [N1N2], N-term detects SETDB2 protein at nucleus by immunofluorescent analysis.Sample: HCT116 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: SETDB2 protein stained by SETDB2 antibody [N1N2], N-term (GTX116932) diluted at 1:500.Blue: Hoechst 33342 staining.Scale bar = 10 £gm.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- SETDB2 antibody immunoprecipitates SETDB2 protein in IP experiments. IP Sample: HeLa whole cell lysate/extract A. 40 £gg HeLa whole cell lysate/extract B. Control with 2 £gg of preimmune rabbit IgG C. Immunoprecipitation of SETDB2 protein by 2 £gg of SETDB2 antibody (GTX116932) 7.5% SDS-PAGE The immunoprecipitated SETDB2 protein was detected by SETDB2 antibody (GTX116932) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.
