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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA5-22274 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CLN2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, A431, H1299, HeLa, HepG2, Molt-4, Raji.
- Concentration
- 0.64 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of CLN2 was performed by separating 30 µg of various whole cell extracts by 7.5% SDS PAGE. Proteins were transferred to a membrane and probed with a CLN2 Polyclonal Antibody (Product # PA5-22274) at a dilution of 1:1000. A: A431, B: H1299.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of CLN2 in methanol-fixed A431 cells using a CLN2 polyclonal antibody (Product # PA5-22274) at a 1:200 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- CLN2 Polyclonal Antibody detects TPP1 protein at cytoplasm on mouse liver by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. CLN2 Polyclonal Antibody (Product # PA5-22274) diluted at 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
