HPA035689

antibody from Atlas Antibodies

Targeting: RFX3

Western blot (Enhanced validation)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Chromatin Immunoprecipitation (Supportive data in Antibodypedia)

Antibody Data

Product number

HPA035689

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#HPA035689, RRID:AB_10671224

Product name

Anti-RFX3

Antibody type

Polyclonal

Description

Polyclonal Antibody against Human RFX3, Gene description: regulatory factor X, 3 (influences HLA class II expression), Validated applications: ChIP, ICC, IHC, WB, Uniprot ID: P48380, Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

100 µl

Concentration

0.1 mg/ml

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Handling

The antibody solution should be gently mixed before use.

References

Submitted references

Airway Basal Stem Cells in COVID-19 Exhibit a Proinflammatory Signature and Impaired Mucocililary Differentiation

Bankoti K, Wang W, Amonkar G, Xiong L, Shui J, Zhao C, Van E, Mwase C, Park J, Mou H, Fang Y, Que J, Bai Y, Lerou P, Ai X
American Journal of Respiratory Cell and Molecular Biology 2024;70(1):26-38

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Loss of RREB1 in pancreatic beta cells reduces cellular insulin content and affects endocrine cell gene expression

Mattis K, Krentz N, Metzendorf C, Abaitua F, Spigelman A, Sun H, Ikle J, Thaman S, Rottner A, Bautista A, Mazzaferro E, Perez-Alcantara M, Manning Fox J, Torres J, Wesolowska-Andersen A, Yu G, Mahajan A, Larsson A, MacDonald P, Davies B, den Hoed M, Gloyn A
Diabetologia 2023;66(4):674-694

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Primary culture of tracheal aspirate-derived human airway basal stem cells

Amonkar G, Wagner R, Bankoti K, Shui J, Ai X, Lerou P
STAR Protocols 2022;3(2):101390

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An essential function for autocrine hedgehog signaling in epithelial proliferation and differentiation in the trachea

Yin W, Liontos A, Koepke J, Ghoul M, Mazzocchi L, Liu X, Lu C, Wu H, Fysikopoulos A, Sountoulidis A, Seeger W, Ruppert C, Günther A, Stainier D, Samakovlis C
Development 2022;149(3)

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Disruption of RFX family transcription factors causes autism, attention-deficit/hyperactivity disorder, intellectual disability, and dysregulated behavior

Harris H, Nakayama T, Lai J, Zhao B, Argyrou N, Gubbels C, Soucy A, Genetti C, Suslovitch V, Rodan L, Tiller G, Lesca G, Gripp K, Asadollahi R, Hamosh A, Applegate C, Turnpenny P, Simon M, Volker-Touw C, Gassen K, Binsbergen E, Pfundt R, Gardeitchik T, Vries B, Immken L, Buchanan C, Willing M, Toler T, Fassi E, Baker L, Vansenne F, Wang X, Ambrus J, Fannemel M, Posey J, Agolini E, Novelli A, Rauch A, Boonsawat P, Fagerberg C, Larsen M, Kibaek M, Labalme A, Poisson A, Payne K, Walsh L, Aldinger K, Balciuniene J, Skraban C, Gray C, Murrell J, Bupp C, Pascolini G, Grammatico P, Broly M, Küry S, Nizon M, Rasool I, Zahoor M, Kraus C, Reis A, Iqbal M, Uguen K, Audebert-Bellanger S, Ferec C, Redon S, Baker J, Wu Y, Zampino G, Syrbe S, Brosse I, Jamra R, Dobyns W, Cohen L, Blomhoff A, Mignot C, Keren B, Courtin T, Agrawal P, Beggs A, Yu T
Genetics in Medicine 2021;23(6):1028-1040

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Dynamic changes in cis-regulatory occupancy by Six1 and its cooperative interactions with distinct cofactors drive lineage-specific gene expression programs during progressive differentiation of the auditory sensory epithelium

Xu P, Shen L, Ding J, Loh Y, Wong E, Xu J, Fritzsch B, Ramakrishnan A, Zhang T, Li J
Nucleic Acids Research 2020;48(6):2880-2896

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Interplay of RFX transcription factors 1, 2 and 3 in motile ciliogenesis

Lemeille S, Paschaki M, Baas D, Morlé L, Duteyrat J, Ait-Lounis A, Barras E, Soulavie F, Jerber J, Thomas J, Zhang Y, Holtzman M, Kistler W, Reith W, Durand B
Nucleic Acids Research 2020;48(16):9019-9036

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Dusp16 Deficiency Causes Congenital Obstructive Hydrocephalus and Brain Overgrowth by Expansion of the Neural Progenitor Pool

Zega K, Jovanovic V, Vitic Z, Niedzielska M, Knaapi L, Jukic M, Partanen J, Friedel R, Lang R, Brodski C
Frontiers in Molecular Neuroscience 2017;10

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GEMC 1 is a critical regulator of multiciliated cell differentiation

Terré B, Piergiovanni G, Segura‐Bayona S, Gil‐Gómez G, Youssef S, Attolini C, Wilsch‐Bräuninger M, Jung C, Rojas A, Marjanović M, Knobel P, Palenzuela L, López‐Rovira T, Forrow S, Huttner W, Valverde M, de Bruin A, Costanzo V, Stracker T
The EMBO Journal 2016;35(9):942-960

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RFX transcription factors are essential for hearing in mice

Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch C, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid C, Ait-Lounis A, Barnes A, Song Y, Eisenman D, Eliyahu E, Frolenkov G, Strome S, Durand B, Zaghloul N, Jones S, Reith W, Hertzano R
Nature Communications 2015;6(1)

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Lineage specificity of primary cilia in the mouse embryo

Bangs F, Schrode N, Hadjantonakis A, Anderson K
Nature Cell Biology 2015;17(2):113-122

Western blot

Enhanced validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Recombinant expression validation

Main image

Experimental details

Western blot analysis in control (vector only transfected HEK293T lysate) and rFX3 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY403344).

Sample type

Human

Protocol

Protocol

Immunocytochemistry

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & vesicles.

Sample type

Human

Immunohistochemistry

Supportive validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Orthogonal validation

Main image

Experimental details

Immunohistochemistry analysis in human testis and skeletal muscle tissues using HPA035689 antibody. Corresponding RFX3 RNA-seq data are presented for the same tissues.

Sample type

Human

Protocol

Protocol

Orthogonal method

Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

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Chromatin Immunoprecipitation

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

ChIP-Exo-Seq composite graph for Anti-RFX3 (HPA035689, Lot 000037835) tested in K562 cells. Strand-specific reads (blue: forward, red: reverse) and IgG controls (black: forward, grey: reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh´s Lab at Cornell University.