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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [3]
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- Product number
- PA5-57366 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- RFX3 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: QYMAMRQQPM QQKQRYKPMQ KVDGVADGFT GSGQQTGTSV EQTVIAQSQH HQQFLDASRA LPEFGEVEIS SLPDGTTFED IKSLQSLYRE HCEAILDVVV NLQFSLIEKL WQTFWRYSPS TPTDGTTITE SSNLSE
- Concentration
- 0.08 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of RFX3 in control (vector only transfected HEK293T lysate) and rFX3 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells). Samples were probed using a RFX3 Polyclonal Antibody (Product # PA5-57366).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of RFX3 in human cell line U-2 OS shows positivity in vesicles & nucleus but excluded from the nucleoli. Samples were probed using a RFX3 Polyclonal Antibody (Product # PA5-57366).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of RFX3 in human testis and liver tissues using RFX3 Polyclonal Antibody (Product # PA5-57366). Corresponding RFX3 RNA-seq data are presented for the same tissues.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of RFX3 in human testis using RFX3 Polyclonal Antibody (Product # PA5-57366) shows high expression.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of RFX3 in human liver using RFX3 Polyclonal Antibody (Product # PA5-57366) shows low expression as expected.
