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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunocytochemistry [2]
- Immunohistochemistry [5]
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- Product number
- PA5-57366 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- RFX3 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Immunogen sequence: QYMAMRQQPM QQKQRYKPMQ KVDGVADGFT GSGQQTGTSV EQTVIAQSQH HQQFLDASRA LPEFGEVEIS SLPDGTTFED IKSLQSLYRE HCEAILDVVV NLQFSLIEKL WQTFWRYSPS TPTDGTTITE SSNLSE Highest antigen sequence identity to the following orthologs: Mouse - 99%, Rat - 100%.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.08 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of RFX3 in human cell line U-2 OS shows positivity in vesicles & nucleus but excluded from the nucleoli. Samples were probed using a RFX3 Polyclonal Antibody (Product # PA5-57366).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorecent analysis of RFX3 in human cell line U-2 OS using RFX3 Polyclonal Antibody (Product # PA5-57366). Staining shows localization to nucleoplasm and vesicles.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of RFX3 in human pancreas using RFX3 Polyclonal Antibody (Product # PA5-57366) shows no positivity in exocrine glandular cells as expected.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of RFX3 in human testis using RFX3 Polyclonal Antibody (Product # PA5-57366) shows high expression.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of RFX3 in human liver using RFX3 Polyclonal Antibody (Product # PA5-57366) shows low expression as expected.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of RFX3 in human skeletal muscle using RFX3 Polyclonal Antibody (Product # PA5-57366) shows no positivity in myocytes as expected.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of RFX3 in human testis and liver tissues using RFX3 Polyclonal Antibody (Product # PA5-57366). Corresponding RFX3 RNA-seq data are presented for the same tissues.
