Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [4]
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Validation data
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- Product number
- NBP1-86301 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-86301, RRID:AB_11019457
- Product name
- Rabbit Polyclonal RFX3 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specificity of human RFX3 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references RFX transcription factors are essential for hearing in mice.
Lineage specificity of primary cilia in the mouse embryo.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications 2015 Oct 15;6:8549
Nature communications 2015 Oct 15;6:8549
Lineage specificity of primary cilia in the mouse embryo.
Bangs FK, Schrode N, Hadjantonakis AK, Anderson KV
Nature cell biology 2015 Feb;17(2):113-22
Nature cell biology 2015 Feb;17(2):113-22
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: RFX3 Antibody [NBP1-86301] - Analysis in control (vector only transfected HEK293T lysate) and rFX3 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: RFX3 Antibody [NBP1-86301] - Staining of human fallopian tube shows strong nuclear positivity in glandular cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: RFX3 Antibody [NBP1-86301] - Staining of human liver shows low expression as expected.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: RFX3 Antibody [NBP1-86301] - Staining in human testis and liver tissues using anti-RFX3 antibody. Corresponding RFX3 RNA-seq data are presented for the same tissues.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: RFX3 Antibody [NBP1-86301] - Staining of human testis shows high expression.