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ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunoprecipitation [1]
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- Product number
- NBP1-50039 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-50039, RRID:AB_10012229
- Product name
- Rabbit Polyclonal CGI-09 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Immunoprecipitation: CGI-09 Antibody [NBP1-50039] - Detection of Human TRM6 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-TRM6 antibody used for IP at 6 mcg/mg lysate. TRM6 was also immunoprecipitated by rabbit anti-TRM6 antibody which recognizes a downstream epitope. For blotting immunoprecipitated TRM6 was used at 1 mcg/ml, and the ReliaBLOT Reagents and Procedures were incorporated. Detection: Chemiluminescence with an exposure time of 10 seconds.
