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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- PAB0542 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB0542, RRID:AB_982725
- Product name
- PKMYT1 (phospho T495) polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic phosphopeptide of PKMYT1.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Keratinocyte G2/M growth arrest by 1,25-dihydroxyvitamin D3 is caused by Cdc2 phosphorylation through Wee1 and Myt1 regulation.
The p53-inducible TSAP6 gene product regulates apoptosis and the cell cycle and interacts with Nix and the Myt1 kinase.
Identification of a consensus motif for Plk (Polo-like kinase) phosphorylation reveals Myt1 as a Plk1 substrate.
Dai X, Yamasaki K, Yang L, Sayama K, Shirakata Y, Tokumara S, Yahata Y, Tohyama M, Hashimoto K
The Journal of investigative dermatology 2004 Jun;122(6):1356-64
The Journal of investigative dermatology 2004 Jun;122(6):1356-64
The p53-inducible TSAP6 gene product regulates apoptosis and the cell cycle and interacts with Nix and the Myt1 kinase.
Passer BJ, Nancy-Portebois V, Amzallag N, Prieur S, Cans C, Roborel de Climens A, Fiucci G, Bouvard V, Tuynder M, Susini L, Morchoisne S, Crible V, Lespagnol A, Dausset J, Oren M, Amson R, Telerman A
Proceedings of the National Academy of Sciences of the United States of America 2003 Mar 4;100(5):2284-9
Proceedings of the National Academy of Sciences of the United States of America 2003 Mar 4;100(5):2284-9
Identification of a consensus motif for Plk (Polo-like kinase) phosphorylation reveals Myt1 as a Plk1 substrate.
Nakajima H, Toyoshima-Morimoto F, Taniguchi E, Nishida E
The Journal of biological chemistry 2003 Jul 11;278(28):25277-80
The Journal of biological chemistry 2003 Jul 11;278(28):25277-80
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- The PKMYT1 (phospho T495) polyclonal antibody (Cat # PAB0542) is used in Western blot to detect Phospho-PKMYT1-T495 in HL-60 (left) and SK-BR-3 (right) cell lysates
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human cancer tissue reacted with PKMYT1 (phospho T495) polyclonal antibody (Cat # PAB0542) which was peroxidase-conjugated to the secondary antibody followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Dot blot analysis of PKMYT1 (phospho T495) polyclonal antibody (Cat # PAB0542) on nitrocellulose membrane. 50 ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5 ug/mL.
- Validation comment
- Dot Blot (Peptide)
