TA500839

antibody from OriGene

Targeting: PKMYT1 MYT1, PPP1R126

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA500839

Provider

OriGene

Proper citation

OriGene Cat#TA500839, RRID:AB_11125667

Product name

PKMYT1 mouse monoclonal antibody, clone OTI2B4 (formerly 2B4)

Antibody type

Monoclonal

Description

PKMYT1 mouse monoclonal antibody, clone OTI2B4 (formerly 2B4)

Host

Mouse

Conjugate

Unconjugated

Epitope

PKMYT1

Isotype

IgG

Antibody clone number

OTI2B4

Vial size

100 µl

Concentration

1.00mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PKMYT1 (RC222657, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PKMYT1.

Validation comment

WB

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-PKMYT1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).(1:200)

Validation comment

WB

Immunocytochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Anti-PKMYT1 mouse monoclonal antibody (TA500839) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PKMYT1(RC222657).

Validation comment

IF

Flow cytometry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Flow cytometric Analysis of Hela cells, using anti-PKMYT1 antibody(TA500839),(Red), compared to a nonspecific negative control antibody,(Blue).

Validation comment

FC

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Flow cytometric Analysis of Jurkat cells, using anti-PKMYT1 antibody(TA500839),(Red), compared to a nonspecific negative control antibody,(Blue).

Validation comment

FC