Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [5]
- Comments [0]
- Validations [0]
Submit
Validation data
Reference
Comment
Report error
- Product number
- ABIN358168 - Provider product page
- Provider
- antibodies-online
- Product name
- anti-Protein Kinase, Membrane Associated tyrosine/threonine 1 (PKMYT1) (pThr495) antibody
- Antibody type
- Polyclonal
- Description
- Protein G affinity chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with control and phosphorylated peptides. The phospho-specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
- Reactivity
- Human
- Host
- Rabbit
- Epitope
- pThr495
- Vial size
- 0.4 mL
- Storage
- Store the antibody undiluted at 2-8°C for one month or (in aliquots) at-20°C for longer.
- Handling
- Avoid repeated freezing and thawing.
Submitted references Keratinocyte G2/M growth arrest by 1,25-dihydroxyvitamin D3 is caused by Cdc2 phosphorylation through Wee1 and Myt1 regulation.
The p53-inducible TSAP6 gene product regulates apoptosis and the cell cycle and interacts with Nix and the Myt1 kinase.
Identification of a consensus motif for Plk (Polo-like kinase) phosphorylation reveals Myt1 as a Plk1 substrate.
Akt inhibits Myt1 in the signalling pathway that leads to meiotic G2/M-phase transition.
Human Myt1 is a cell cycle-regulated kinase that inhibits Cdc2 but not Cdk2 activity.
Dai X, Yamasaki K, Yang L, Sayama K, Shirakata Y, Tokumara S, Yahata Y, Tohyama M, Hashimoto K
The Journal of investigative dermatology 2004 Jun;122(6):1356-64
The Journal of investigative dermatology 2004 Jun;122(6):1356-64
The p53-inducible TSAP6 gene product regulates apoptosis and the cell cycle and interacts with Nix and the Myt1 kinase.
Passer BJ, Nancy-Portebois V, Amzallag N, Prieur S, Cans C, Roborel de Climens A, Fiucci G, Bouvard V, Tuynder M, Susini L, Morchoisne S, Crible V, Lespagnol A, Dausset J, Oren M, Amson R, Telerman A
Proceedings of the National Academy of Sciences of the United States of America 2003 Mar 4;100(5):2284-9
Proceedings of the National Academy of Sciences of the United States of America 2003 Mar 4;100(5):2284-9
Identification of a consensus motif for Plk (Polo-like kinase) phosphorylation reveals Myt1 as a Plk1 substrate.
Nakajima H, Toyoshima-Morimoto F, Taniguchi E, Nishida E
The Journal of biological chemistry 2003 Jul 11;278(28):25277-80
The Journal of biological chemistry 2003 Jul 11;278(28):25277-80
Akt inhibits Myt1 in the signalling pathway that leads to meiotic G2/M-phase transition.
Okumura E, Fukuhara T, Yoshida H, Hanada Si S, Kozutsumi R, Mori M, Tachibana K, Kishimoto T
Nature cell biology 2002 Feb;4(2):111-6
Nature cell biology 2002 Feb;4(2):111-6
Human Myt1 is a cell cycle-regulated kinase that inhibits Cdc2 but not Cdk2 activity.
Booher RN, Holman PS, Fattaey A
The Journal of biological chemistry 1997 Aug 29;272(35):22300-6
The Journal of biological chemistry 1997 Aug 29;272(35):22300-6
No comments: Submit comment
No validations: Submit validation data