HPA044833

antibody from Atlas Antibodies

Targeting: U2AF1 RN, RNU2AF1, U2AF35, U2AFBP

Western blot (Enhanced validation)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

HPA044833

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#HPA044833, RRID:AB_10960990

Product name

Anti-U2AF1

Antibody type

Polyclonal

Description

Polyclonal Antibody against Human U2AF1, Gene description: U2 small nuclear RNA auxiliary factor 1, Alternative Gene Names: RN, RNU2AF1, U2AF35, U2AFBP, Validated applications: IHC, WB, ICC, Uniprot ID: Q01081, Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

100 µl

Concentration

0.5 mg/ml

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Handling

The antibody solution should be gently mixed before use.

References

Submitted references

IKKβ is required for the formation of the NLRP3 inflammasome

Nanda S, Prescott A, Figueras‐Vadillo C, Cohen P
EMBO reports 2021;22(10)

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RNA- and Antibody-Based Profiling of the Human Proteome with Focus on Chromosome 19

Stadler C, Fagerberg L, Sivertsson Å, Oksvold P, Zwahlen M, Hallström B, Lundberg E, Uhlén M
Journal of Proteome Research 2014;13(4):2019-2027

Western blot

Enhanced validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Western blot analysis in U2OS cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-U2AF1 antibody. Remaining relative intensity is presented.

Sample type

Human

Protocol

Protocol

Immunocytochemistry

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.

Sample type

Human