Antibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [1]
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Validation data
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- Product number
- NBP2-59375 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal ATP7b Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified. Detects 160kDa in rat brain membrane preparations.
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 100 ug
- Storage
- Store at -20C.
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Western Blot: ATP7b Antibody (S62-29) [NBP2-59375] - Analysis of Rat Brain Membrane showing detection of ~160 kDa Copper Transporting ATPase 2 protein using Mouse Anti-Copper Transporting ATPase 2 Monoclonal Antibody, Clone S62-29. Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Brain Membrane cell lysate. Load: 20 ug. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-Copper Transporting ATPase 2 Monoclonal Antibody at 1:1000 for 16 hours at 4 degrees C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:100 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~160 kDa.