Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
- Other assay [1]
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Validation data
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- Product number
- A302-321A - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PHF23 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- The recommended shelf life for this product is 1 year from date of receipt.
- Concentration
- 1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human PHF23 by western blot. Samples: Whole cell lysate (5, 15, and 50 µg) from HeLa cells. Antibody: Affinity purified rabbit anti-PHF23 antibody (Product # A302-321A; lot A302-321A-1) used at 0.1 µg/mL. Detection: Chemiluminescence with an exposure time of 30 seconds.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human PHF23 by western blot and immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-PHF23 antibody A302-321A used for WB at 0.1 µg/ml (A) and 0.4 µg/ml (B) and used for IP at 10 µg/mg lysate. PHF23 was also immunoprecipitated by rabbit anti-PHF23 antibody A302-320A, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A and B).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human PHF23 by immunohistochemistry. Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti- PHF23 (Cat. No. A302-321A Lot1) used at a dilution of 1:1,000 (1µg/ml). Detection: DAB.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human PHF23 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-PHF23 antibody (Product # A302-321A; lot A302-321A-1) used for IP at 10 µg/mg lysate. PHF23 was also immunoprecipitated by rabbit anti-PHF23 antibody (Product # A302-320A), which recognizes an upstream epitope. For blotting immunoprecipitated PHF23 (Product # A302-321A) was used at 1.0 µg/mL. Detection: Chemiluminescence with exposure time of 30 seconds.