Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- NBP1-28747 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-28747, RRID:AB_1913972
- Product name
- Rabbit Polyclonal PHF23 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PHF23 Antibody [NBP1-28747] - Samples: Whole cell lysate (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-PHF23 antibody NBP1-28747 used for WB at 0.1 ug/ml (A) and 0.4 ug/ml (B) and used for IP at 10 ug/mg lysate. PHF23 was also immunoprecipitated by rabbit anti-PHF23 antibody NBP1-28746, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A and B).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: PHF23 Antibody [NBP1-28747] - Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti- PHF23 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB.